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Scientific article
English

Molecular Weight of RNA Subunits of Rous Sarcoma Virus Determined by Electron Microscopy

Published inJournal of Virology, vol. 15, no. 1, p. 161-166
Publication date1975
Abstract

Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 μCi of [³²P]orthophosphate per ml. Virus was collected by centrifugation, and the RNA was extracted and denatured with dimethyl sulfoxide, and the 33S subunit RNA was isolated by sucrose gradient centrifugation. The molecular weight of the RSV subunit RNA was determined by length measurement in the electron microscope, by using bacteriophage MS2 RNA as a length marker. Molecules of between 2.5 and 3.3 μm in length made up over 50% of the subunit RNA preparation. In this paper, we define RSV RNA subunits as that RNA released from the 70S RNA complex by dimethyl sulfoxide treatment, which sediments as a peak at 33S. Assuming the molecular weight of MS2 RNA to be 1.2 times 10⁶, we calculate the molecular weight of RSV subunit RNA to be 3.12 times 10⁶ plus or minus 0.25 times 10⁶.

Keywords
  • Animals
  • Avian sarcoma viruses/analysis
  • Density gradient centrifugation
  • Chick embryo
  • Culture techniques
  • Dimethyl sulfoxide
  • Fibroblasts
  • Electron microscopy
  • Molecular weight
  • Nucleic acid denaturation
  • Phosphates
  • Phosphorus radioisotopes
  • Viral RNA/analysis
Funding
  • Swiss National Science Foundation - 3.096.73 et 3.810.72
Citation (ISO format)
JACOBSON, Ann Béatrice, BROMLEY, Peter A. Molecular Weight of RNA Subunits of Rous Sarcoma Virus Determined by Electron Microscopy. In: Journal of Virology, 1975, vol. 15, n° 1, p. 161–166.
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Article (Published version)
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Identifiers
  • PID : unige:128117
  • PMID : 163340
ISSN of the journal0022-538X
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