Molecular Weight of RNA Subunits of Rous Sarcoma Virus Determined by Electron Microscopy

Article Article scientifique Article Molecular Weight of RNA Subunits of Rous Sarcoma Virus Determined by Electron Microscopy eng 1975 author Jacobson Ann Béatrice 62653 author Bromley Peter A. 161-166 10.1128/JVI.15.1.161-166.1975 163340 0022-538X Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 μCi of [³²P]orthophosphate per ml. Virus was collected by centrifugation, and the RNA was extracted and denatured with dimethyl sulfoxide, and the 33S subunit RNA was isolated by sucrose gradient centrifugation. The molecular weight of the RSV subunit RNA was determined by length measurement in the electron microscope, by using bacteriophage MS2 RNA as a length marker. Molecules of between 2.5 and 3.3 μm in length made up over 50% of the subunit RNA preparation. In this paper, we define RSV RNA subunits as that RNA released from the 70S RNA complex by dimethyl sulfoxide treatment, which sediments as a peak at 33S. Assuming the molecular weight of MS2 RNA to be 1.2 times 10⁶, we calculate the molecular weight of RSV subunit RNA to be 3.12 times 10⁶ plus or minus 0.25 times 10⁶. Animals Avian sarcoma viruses/analysis Density gradient centrifugation Chick embryo Culture techniques Dimethyl sulfoxide Fibroblasts Electron microscopy Molecular weight Nucleic acid denaturation Phosphates Phosphorus radioisotopes Viral RNA/analysis Dewey 570 Swiss National Science Foundation 3.096.73 et 3.810.72 CH 43 Département de biologie moléculaire 161 false Journal of Virology 15 1 52239c1d-5c95-487a-a182-6f4f75c46207 Article (Published version) Restricted jvirol00229-0171.pdf 2155871 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

Title	Published in	Year	Views	Downloads

Studies on secondary structure of single-stranded RNA from bacteriophage MS2 by electron microscopy	Proceedings of the National Academy of Sciences	1976	299	173
Molecular Weight of RNA Subunits of Rous Sarcoma Virus Determined by Electron Microscopy	Journal of Virology	1975	286	0

Archive ouverte UNIGE

Jacobson, Ann Béatrice