UNIGE document Scientific Article
previous document  unige:12762  next document
add to browser collection
Title

Simultaneous quantification of ten cytotoxic drugs by a validated LC-ESI-MS/MS method

Authors
Fleury-Souverain, Sandrine
Antinori, Paola
Published in Analytical and Bioanalytical Chemistry. 2010, vol. 398, no. 7-8, p. 3033-42
Abstract A liquid chromatography separation with electrospray ionisation and tandem mass spectrometry detection method was developed for the simultaneous quantification of ten commonly handled cytotoxic drugs in a hospital pharmacy. These cytotoxic drugs are cytarabine, gemcitabine, methotrexate, etoposide phosphate, cyclophosphamide, ifosfamide, irinotecan, doxorubicin, epirubicin and vincristine. The chromatographic separation was carried out by RPLC in less than 21 min, applying a gradient elution of water and acetonitrile in the presence of 0.1% formic acid. MS/MS was performed on a triple quadrupole in selected reaction monitoring mode. The analytical method was validated to determine the limit of quantification (LOQ) and quantitative performance: lowest LOQs were between 0.25 and 2 ng mL(-1) for the ten investigated cytotoxic drugs; trueness values (i.e. recovery) were between 85% and 110%, and relative standard deviations for both repeatability and intermediate precision were always inferior to 15%. The multi-compound method was successfully applied for the quality control of pharmaceutical formulations and for analyses of spiked samples on potentially contaminated surfaces.
Keywords CytotoxicAntineoplastic drugsLC–MS/MSPharmaceutical formulationSRMValidation
Stable URL https://archive-ouverte.unige.ch/unige:12762
Full text
Identifiers
PMID: 20927508
Structures
Research group Chimie et protéomique clinique (270)

198 hits

1 download

Update

Deposited on : 2010-12-02

Export document
Format :
Citation style :