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Title

Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex

Authors
Fiumelli, Hubert
Tyagarajan, Shiva
Published in Brain Structure and Function. 2018, vol. 223, no. 4, p. 1999-2012
Abstract Inhibitory control of pyramidal neurons plays a major role in governing the excitability in the brain. While spatial mapping of inhibitory inputs onto pyramidal neurons would provide important structural data on neuronal signaling, studying their distribution at the single cell level is difficult due to the lack of easily identifiable anatomical proxies. Here, we describe an approach where in utero electroporation of a plasmid encoding for fluorescently tagged gephyrin into the precursors of pyramidal cells along with ionotophoretic injection of Lucifer Yellow can reliably and specifically detect GABAergic synapses on the dendritic arbour of single pyramidal neurons. Using this technique and focusing on the basal dendritic arbour of layer 2/3 pyramidal cells of the medial prefrontal cortex, we demonstrate an intense development of GABAergic inputs onto these cells between postnatal days 10 and 20. While the spatial distribution of gephyrin clusters was not affected by the distance from the cell body at postnatal day 10, we found that distal dendritic segments appeared to have a higher gephyrin density at later developmental stages. We also show a transient increase around postnatal day 20 in the percentage of spines that are carrying a gephyrin cluster, indicative of innervation by a GABAergic terminal. Since the precise spatial arrangement of synaptic inputs is an important determinant of neuronal responses, we believe that the method described in this work may allow a better understanding of how inhibition settles together with excitation, and serve as basics for further modelling studies focusing on the geometry of dendritic inhibition during development.
Keywords Age FactorsAnimalsNewbornCarrier Proteins/metabolismDendrites/metabolismDendritic Spines/physiologyEmbryoMammalianGene Expression RegulationDevelopmental/physiologyIn Vitro TechniquesIsoquinolines/metabolismMembrane Proteins/metabolismMicroscopyConfocalNeural Inhibition/physiologyNeurogenesis/physiologyPrefrontal Cortex/cytology/diagnostic imaging/growth & developmentPyramidal Cells/physiologyRatsWistarSynapses/physiologyTime FactorsVesicular Inhibitory Amino Acid Transport Proteins/metabolismGamma-Aminobutyric Acid/metabolism
Identifiers
PMID: 29322238
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Article (Published version) (5.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Anesthésie et neurosciences (883)
Traitement et neurobiologie de la dépression (916)
Project FNS: 31003A- 130625 (to LV)
Citation
(ISO format)
VIRTANEN, Mari et al. Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex. In: Brain Structure and Function, 2018, vol. 223, n° 4, p. 1999-2012. doi: 10.1007/s00429-017-1602-0 https://archive-ouverte.unige.ch/unige:126964

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Deposited on : 2019-11-26

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