Scientific article

Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex

Published inBrain Structure and Function, vol. 223, no. 4, p. 1999-2012
Publication date2018

Inhibitory control of pyramidal neurons plays a major role in governing the excitability in the brain. While spatial mapping of inhibitory inputs onto pyramidal neurons would provide important structural data on neuronal signaling, studying their distribution at the single cell level is difficult due to the lack of easily identifiable anatomical proxies. Here, we describe an approach where in utero electroporation of a plasmid encoding for fluorescently tagged gephyrin into the precursors of pyramidal cells along with ionotophoretic injection of Lucifer Yellow can reliably and specifically detect GABAergic synapses on the dendritic arbour of single pyramidal neurons. Using this technique and focusing on the basal dendritic arbour of layer 2/3 pyramidal cells of the medial prefrontal cortex, we demonstrate an intense development of GABAergic inputs onto these cells between postnatal days 10 and 20. While the spatial distribution of gephyrin clusters was not affected by the distance from the cell body at postnatal day 10, we found that distal dendritic segments appeared to have a higher gephyrin density at later developmental stages. We also show a transient increase around postnatal day 20 in the percentage of spines that are carrying a gephyrin cluster, indicative of innervation by a GABAergic terminal. Since the precise spatial arrangement of synaptic inputs is an important determinant of neuronal responses, we believe that the method described in this work may allow a better understanding of how inhibition settles together with excitation, and serve as basics for further modelling studies focusing on the geometry of dendritic inhibition during development.

  • Age Factors
  • Animals
  • Newborn
  • Carrier Proteins/metabolism
  • Dendrites/metabolism
  • Dendritic Spines/physiology
  • Embryo
  • Mammalian
  • Gene Expression Regulation
  • Developmental/physiology
  • In Vitro Techniques
  • Isoquinolines/metabolism
  • Membrane Proteins/metabolism
  • Microscopy
  • Confocal
  • Neural Inhibition/physiology
  • Neurogenesis/physiology
  • Prefrontal Cortex/cytology/diagnostic imaging/growth & development
  • Pyramidal Cells/physiology
  • Rats
  • Wistar
  • Synapses/physiology
  • Time Factors
  • Vesicular Inhibitory Amino Acid Transport Proteins/metabolism
  • Gamma-Aminobutyric Acid/metabolism
  • Swiss National Science Foundation - 31003A- 130625 (to LV)
Citation (ISO format)
VIRTANEN, Mari et al. Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex. In: Brain Structure and Function, 2018, vol. 223, n° 4, p. 1999–2012. doi: 10.1007/s00429-017-1602-0
Main files (1)
Article (Published version)
ISSN of the journal1863-2653

Technical informations

Creation11/05/2019 11:51:00 AM
First validation11/05/2019 11:51:00 AM
Update time03/15/2023 6:27:13 PM
Status update03/15/2023 6:27:12 PM
Last indexation10/19/2023 11:39:55 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack