Scientific Article - Review
previous document  unige:124452  next document
add to browser collection

Enzymatic complexes across scales

Kastritis, Panagiotis L
Published in Essays in Biochemistry. 2018, vol. 62, no. 4, p. 501-514
Abstract An unprecedented opportunity to integrate ~100 years of meticulous in vitro biomolecular research is currently provided in the light of recent advances in methods to visualize closer-to-native architectures of biomolecular machines, and metabolic enzymes in particular. Traditional views of enzymes, namely biomolecular machines, only partially explain their role, organization and kinetics in the cellular milieu. Enzymes self- or hetero-associate, form fibers, may bind to membranes or cytoskeletal elements, have regulatory roles, associate into higher order assemblies (metabolons) or even actively participate in phase-separated membraneless organelles, and all the above in a transient, temporal and spatial manner in response to environmental changes or structural/functional changes of their assemblies. Here, we focus on traditional and emerging concepts in cellular biochemistry and discuss new opportunities in bridging structural, molecular and cellular analyses for metabolic pathways, accumulated over the years, highlighting functional aspects of enzymatic complexes discussed across different levels of spatial resolution.
Keywords Electron microscopyEnzymologyGlycolysisProtein-protein interactions
PMID: 30315098
Full text
Research group Métabolisme des lipides (1001)
(ISO format)
KASTRITIS, Panagiotis L, GAVIN PERRIN, Anne-Claude. Enzymatic complexes across scales. In: Essays in Biochemistry, 2018, vol. 62, n° 4, p. 501-514. doi: 10.1042/EBC20180008

131 hits



Deposited on : 2019-10-15

Export document
Format :
Citation style :