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Quantification of cyclosporine and tacrolimus in whole blood. Comparison of liquid chromatography-electrospray mass spectrometry with the enzyme multiplied immunoassay technique

Published in Clinical biochemistry. 2008, vol. 41, no. 10-11, p. 910-3
Abstract OBJECTIVES: The aim of this work was to compare a validated liquid chromatography-mass spectrometry (LC-MS) method with the commercial enzyme multiplied immunoassay technique (EMIT) for cyclosporine and tacrolimus whole blood quantification. DESIGN AND METHODS: Samples of transplant patients receiving cyclosporine (n=38) or tacrolimus (n=41) were analyzed successively by LC-MS and EMIT. Several statistical approaches for method comparison were evaluated and Passing-Bablok and Bland-Altman analyses chosen. RESULTS: Overestimations of the concentrations measured with EMIT compared to LC-MS were observed with means of 23% (range: 6% to 46%) for cyclosporine and 30% (range: -3% to 73%) for tacrolimus. CONCLUSION: The EMIT demonstrated significant positive biases due to cross-reactions with metabolites. This indicates that, in some clinical situations, a selective method such as LC-MS is preferable for therapeutic drug monitoring in transplant patients.
Keywords Cross ReactionsCyclosporine/bloodEnzyme Multiplied Immunoassay TechniqueHumansImmunoassaySpectrometry, Mass, Electrospray Ionization/methodsTacrolimus/bloodTransplantation Immunology
Stable URL https://archive-ouverte.unige.ch/unige:1238
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PMID: 18358833

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Deposited on : 2009-03-27

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