Molecular chaperones are abundant and essential molecular machines that support cellular proteostasis through the folding and structural maintanance of proteins. The heat-shock protein 90 family of proteins is an evolutionarily conserved class of molecular chaperones, important in the structural maintenance of hundreds of substrate proteins. In this work, we have investigated the nuclear localization of nucleocytoplasmic Hsp90 isoforms and the function of the mitochondrial isoform TRAP1 as a molecular chaperone.