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Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence

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Published in Journal of Chromatography. A. 2008, vol. 1204, no. 2, p. 183-90
Abstract Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample.
Keywords Body Fluids/chemistryElectrophoresis, Capillary/methodsHumansHydrogen-Ion ConcentrationLasersPharmaceutical Preparations/analysis/bloodPlasma/chemistryPropranolol/analysis/bloodReference StandardsReproducibility of ResultsSensitivity and SpecificitySpectrometry, Fluorescence/methodsTemperature
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PMID: 18468608
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SCHAPPLER, Julie et al. Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence. In: Journal of Chromatography. A, 2008, vol. 1204, n° 2, p. 183-90. https://archive-ouverte.unige.ch/unige:1236

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Deposited on : 2009-03-27

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