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Title

Use of Amplified Fragment Length Polymorphism in Molecular Typing of Legionella pneumophila and Application to Epidemiological Studies

Authors
Valsangiacomo, Claudio
Balmelli, Tiziano
Published in Journal of Clinical Microbiology. 1995, vol. 33, no. 7, p. 1716-1719
Abstract A novel method for molecular typing of organisms, amplified fragment length polymorphism analysis, was tested for its suitability in epidemiological studies in medical microbiology. Amplified fragment length polymorphism analysis, originally developed for typing crop plants, consists of a simple restriction-ligation reaction and a subsequent PCR amplification. In a single-step reaction, the genomic DNA is digested and the restriction fragments are ligated to specially constructed adapters. PCR amplification of such tagged restriction fragments with primers complementary to the adapters allows the detection of restriction fragment length polymorphisms upon resolution on agarose gels. The method is fast, efficient, and reproducible for typing strains of Legionella pneumophila isolated from both humans and the environment. The accuracy of the method was tested by comparison with standard restriction fragment length polymorphism typing performed with both a ribosomal and a genomic probe.
Keywords Bacterial Typing TechniquesBase SequenceDNA PrimersGeneticsBacterial DNAGene AmplificationGenetic VariationHumansLegionella pneumophilaClassificationIsolation & purificationMolecular EpidemiologyMolecular Sequence DataPolymerase Chain ReactionRestriction Fragment Length Polymorphism
Identifiers
PMID: 7665634
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VALSANGIACOMO, Claudio et al. Use of Amplified Fragment Length Polymorphism in Molecular Typing of Legionella pneumophila and Application to Epidemiological Studies. In: Journal of Clinical Microbiology, 1995, vol. 33, n° 7, p. 1716-1719. https://archive-ouverte.unige.ch/unige:123120

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Deposited on : 2019-09-13

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