Use of Amplified Fragment Length Polymorphism in Molecular Typing of <i>Legionella pneumophila</i> and Application to Epidemiological Studies

Article Article scientifique Article Use of Amplified Fragment Length Polymorphism in Molecular Typing of <i>Legionella pneumophila</i> and Application to Epidemiological Studies eng 1995 author Valsangiacomo Claudio author Baggi Franca 503730 author Gaia Valeria 83030 author Balmelli Tiziano author Peduzzi Raffael 24692 author Piffaretti Jean-Claude 24760 1716-1719 7665634 0095-1137 A novel method for molecular typing of organisms, amplified fragment length polymorphism analysis, was tested for its suitability in epidemiological studies in medical microbiology. Amplified fragment length polymorphism analysis, originally developed for typing crop plants, consists of a simple restriction-ligation reaction and a subsequent PCR amplification. In a single-step reaction, the genomic DNA is digested and the restriction fragments are ligated to specially constructed adapters. PCR amplification of such tagged restriction fragments with primers complementary to the adapters allows the detection of restriction fragment length polymorphisms upon resolution on agarose gels. The method is fast, efficient, and reproducible for typing strains of Legionella pneumophila isolated from both humans and the environment. The accuracy of the method was tested by comparison with standard restriction fragment length polymorphism typing performed with both a ribosomal and a genomic probe. Bacterial Typing Techniques Base Sequence DNA Primers Genetics Bacterial DNA Gene Amplification Genetic Variation Humans Legionella pneumophila Classification Isolation & purification Molecular Epidemiology Molecular Sequence Data Polymerase Chain Reaction Restriction Fragment Length Polymorphism true Journal of Clinical Microbiology 33 7 dd8228b3-3196-4db9-a4e0-d39d8d3e14b3 Article (Published version) Restricted 331716.pdf 326781 application/pdf restrictedAccess

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Use of Amplified Fragment Length Polymorphism in Molecular Typing of Legionella pneumophila and Application to Epidemiological Studies	Journal of Clinical Microbiology			1995	280	0

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Baggi, Franca