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Scientific article
English

Trypsin immobilization on an ethylenediamine-based monolithic minidisk for rapid on-line peptide mass fingerprinting studies

Published inJournal of chromatography, vol. 1216, no. 13, p. 2695-2699
Publication date2009
Abstract

The aim of this work was to develop a trypsin-based micro-immobilized enzyme reactor prepared on a monolithic ethylenediamine BIA Separations CIM (convective interaction media) minidisk. The micro-immobilized enzyme reactor (IMER) was integrated in a liquid chromatography system hyphenated to electrospray ionization tandem mass spectrometry to carry out on-line protein digestion and identification. The performance of this IMER was compared with that obtained using a previously developed bioreactor prepared on a conventional CIM ethylenediamine disk and with that of the commercially available Poroszyme immobilized trypsin cartridge. In this work, we showed how different proteins were identified with good recoveries using a digestion time of 10min only.

Keywords
  • Immobilized enzyme reactor
  • Immobilized trypsin
  • CIM disks
  • Minidisks
Citation (ISO format)
NICOLI, Raul et al. Trypsin immobilization on an ethylenediamine-based monolithic minidisk for rapid on-line peptide mass fingerprinting studies. In: Journal of chromatography, 2009, vol. 1216, n° 13, p. 2695–2699. doi: 10.1016/j.chroma.2008.10.046
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ISSN of the journal0021-9673
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Creation03/20/2009 11:57:00 AM
First validation03/20/2009 11:57:00 AM
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