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Trypsin immobilization on an ethylenediamine-based monolithic minidisk for rapid on-line peptide mass fingerprinting studies

Stella, Cinzia
Published in Journal of Chromatography. A. 2009, vol. 1216, no. 13, p. 2695-2699
Abstract The aim of this work was to develop a trypsin-based micro-immobilized enzyme reactor prepared on a monolithic ethylenediamine BIA Separations CIM (convective interaction media) minidisk. The micro-immobilized enzyme reactor (IMER) was integrated in a liquid chromatography system hyphenated to electrospray ionization tandem mass spectrometry to carry out on-line protein digestion and identification. The performance of this IMER was compared with that obtained using a previously developed bioreactor prepared on a conventional CIM ethylenediamine disk and with that of the commercially available Poroszyme immobilized trypsin cartridge. In this work, we showed how different proteins were identified with good recoveries using a digestion time of 10min only.
Keywords Immobilized enzyme reactorImmobilized trypsinCIM disksMinidisks
Stable URL https://archive-ouverte.unige.ch/unige:1213
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PMID: 18962647

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Deposited on : 2009-03-23

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