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Title

Determination of suxamethonium in a pharmaceutical formulation by capillary electrophoresis with contactless conductivity detection (CE-C(4)D)
Authors
Nussbaumer, Susanne
Fleury-Souverain, Sandrine
Rudaz, Serge
Bonnabry, Pascal
Veuthey, Jean-Luc
Published in Journal of Pharmaceutical and Biomedical Analysis. 2009, vol. 49, no. 2, p. 333-7
Abstract A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of suxamethonium (SUX) in a pharmaceutical formulation. A hydro-organic mixture, consisting of 100mM Tris-acetate buffer at pH 4.2 and acetonitrile (90:10, v/v), was selected as background electrolyte (BGE). The applied voltage was 30kV, and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50mum and a total length of 64.5cm. Under these conditions, a complete separation between SUX, sodium ions and the main degradation products (choline) was achieved in less than 4min. The presence of acetonitrile in the BGE allowed a reduction of SUX adsorption on the capillary wall. The CE-C(4)D method was validated, and trueness values between 98.8% and 101.1% were obtained with repeatability and intermediate precision values of 0.7-1.3% and 1.2-1.6%, respectively. Therefore, this method was found appropriate for controlling pharmaceutical formulations containing suxamethonium and degradation products.
Keywords Capacitively coupled contactless — Conductivity detection — Capillary electrophoresis — Succinylcholine chloride — Suxamethonium — Validation
Stable URL https://archive-ouverte.unige.ch/unige:1212
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Identifiers
PMID: 19121913
Structures
Faculté des sciences / Section des sciences pharmaceutiques

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Deposited on : 2009-03-23

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