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Scientific article
English

Determination of suxamethonium in a pharmaceutical formulation by capillary electrophoresis with contactless conductivity detection (CE-C(4)D)

Published inJournal of pharmaceutical and biomedical analysis, vol. 49, no. 2, p. 333-337
Publication date2009
Abstract

A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of suxamethonium (SUX) in a pharmaceutical formulation. A hydro-organic mixture, consisting of 100mM Tris-acetate buffer at pH 4.2 and acetonitrile (90:10, v/v), was selected as background electrolyte (BGE). The applied voltage was 30kV, and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50mum and a total length of 64.5cm. Under these conditions, a complete separation between SUX, sodium ions and the main degradation products (choline) was achieved in less than 4min. The presence of acetonitrile in the BGE allowed a reduction of SUX adsorption on the capillary wall. The CE-C(4)D method was validated, and trueness values between 98.8% and 101.1% were obtained with repeatability and intermediate precision values of 0.7-1.3% and 1.2-1.6%, respectively. Therefore, this method was found appropriate for controlling pharmaceutical formulations containing suxamethonium and degradation products.

Keywords
  • Capacitively coupled contactless
  • Conductivity detection
  • Capillary electrophoresis
  • Succinylcholine chloride
  • Suxamethonium
  • Validation
Citation (ISO format)
NUSSBAUMER, Susanne et al. Determination of suxamethonium in a pharmaceutical formulation by capillary electrophoresis with contactless conductivity detection (CE-C(4)D). In: Journal of pharmaceutical and biomedical analysis, 2009, vol. 49, n° 2, p. 333–337. doi: 10.1016/j.jpba.2008.11.018
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ISSN of the journal0731-7085
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Creation02/25/2009 9:40:00 AM
First validation02/25/2009 9:40:00 AM
Update time03/14/2023 3:03:36 PM
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