AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting

Keszei, Zsofia; Picard, Didier

doi:10.24450/journals/abrep.2019.e25

Scientific article

English

AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting

ContributorsKeszei, Zsofia; Picard, Didier

Published inAntibody Reports, vol. 2, no. e25

Publication date2019

Abstract

The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.

Keywords

HA tag
12CA5
Western blot

Affiliation entities

Faculté des sciences / Section de biologie / Département de biologie cellulaire

Citation (ISO format)

KESZEI, Zsofia, PICARD, Didier. AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting. In: Antibody Reports, 2019, vol. 2, n° e25. doi: 10.24450/journals/abrep.2019.e25

Article (Published version)

CC BY

Identifiers

PID : unige:117129
DOI : 10.24450/journals/abrep.2019.e25

Journal ISSN2624-8557

537views

248downloads

Creation08/05/2019 15:06:00

First validation08/05/2019 15:06:00

Update15/03/2023 16:29:36

Status update15/03/2023 16:29:35

Last indexation31/10/2024 13:26:00

Archive ouverte UNIGE

AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting

The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.

Technical informations