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CRISPR/Cas9 Genome Editing in Embryonic Stem Cells

Spielmann, Malte
Published in Methods in Molecular Biology. 2017, vol. 1468, p. 221-34
Abstract Targeted mutagenesis is required to evaluate the function of DNA segments across the genome. In recent years the CRISPR/Cas9 technology has been widely used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects. CRISPR/Cas9-derived tools indeed allow the production of a wide-range of engineered mutations: from point mutations to large chromosomal rearrangements such as deletions, duplications and inversions. Here we present a protocol to engineer Embryonic Stem Cells (ESC) with desired mutations using transfection of custom-made CRISPR/Cas9 vectors. These methods allow the in vivo modeling of congenital mutations and the functional interrogation of DNA sequences.
Keywords AnimalsCRISPR-Cas SystemsGene EditingGenetic EngineeringMiceModelsGeneticMouse Embryonic Stem Cells/cytologyMutationTransfection
PMID: 27662879
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Research group Contrôle épigénétique du développement embryonnaire (999)
(ISO format)
ANDREY, Guillaume, SPIELMANN, Malte. CRISPR/Cas9 Genome Editing in Embryonic Stem Cells. In: Methods in Molecular Biology, 2017, vol. 1468, p. 221-34. doi: 10.1007/978-1-4939-4035-6_15

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Deposited on : 2019-04-30

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