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Resveratrol long-term treatment differentiates INS-1E beta-cell towards improved glucose response and insulin secretion

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Published in Pflügers Archiv. 2019, vol. 471, no. 2, p. 337-345
Abstract The clonal INS-1E beta-cell line has proven to be instrumental for numerous studies investigating the mechanisms of glucose-stimulated insulin secretion. The composition of its culture medium has not changed over the years, although some compounds have been recently highlighted for their effects on tissue differentiation. The present study investigated the effects of long-term treatment of INS-1E cells with 1 μM resveratrol on glucose-stimulated insulin secretion, testing an extended glucose dose response. The data demonstrate that chronic exposure to low-dose resveratrol expands the range of the glucose dose response of INS-1E cells beyond 15 mM glucose. We also assessed whether such beneficial effects could be retained after resveratrol withdrawal from the culture medium. This was not the case as INS-1E cells deprived of resveratrol returned to the phenotype of naïve cells, i.e., exhibiting a plateau phase at 15 mM glucose. Of note, although resveratrol has antioxidant properties, it cannot substitute for β-mercaptoethanol normally present in the medium of INS-1E cells as a reducing agent. In conclusion, the addition of resveratrol as a standard component of the culture medium of INS-1E cells improves glucose-stimulated insulin secretion.
Keywords Pancreatic beta-cellInsulin secretionResveratrolINS-1E cells
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PMID: 30310992
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Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Mitochondries et sécrétion d'insuline (671)
Projects FNS: 146984
FNS: 166625
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CASIMIR, Marina Shamini, CHAFFARD, Gaelle, MAECHLER, Pierre. Resveratrol long-term treatment differentiates INS-1E beta-cell towards improved glucose response and insulin secretion. In: Pflügers Archiv, 2019, vol. 471, n° 2, p. 337-345. https://archive-ouverte.unige.ch/unige:116615

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Deposited on : 2019-04-24

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