Scientific article

Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes

Published inJournal of neuroimmunology, vol. 126, no. 1-2, p. 86-98
Publication date2002

We examined the potential expression and function of alpha7 nicotinic acetylcholine receptors (nAChRs) in leukocytes. RT-PCR with alpha7 specific primers revealed the presence of the receptor mRNA in leukocytes. Immunoblotting and immunofluorescence experiments demonstrated the expression of a protein that is recognized by alpha7 specific antibodies. However, nicotine and acetylcholine (ACh) failed to elicit current in leukocytes. Binding experiments with alpha-bungarotoxin rhodamine conjugated were negative, illustrating the absence of a high-affinity binding site. RT-PCR analysis revealed the selective expression of the dupalpha7 mRNA. These data indicate that leukocytes express in their membrane the dupalpha7 protein but its physiological role remains to be identified.

  • Antibody Specificity
  • Blotting, Western
  • Bungarotoxins/metabolism/pharmacology
  • Calcium/metabolism
  • Cytosol/metabolism
  • Electrophysiology
  • Fluorescent Dyes
  • Gene Expression/immunology
  • HL-60 Cells
  • Humans
  • Leukocytes/ chemistry/ physiology
  • Membrane Potentials/physiology
  • RNA, Messenger/analysis
  • Receptors, Nicotinic/ analysis/ genetics/immunology
  • Rhodamines
Affiliation Not a UNIGE publication
Citation (ISO format)
VILLIGER, Y. et al. Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes. In: Journal of neuroimmunology, 2002, vol. 126, n° 1-2, p. 86–98.
Main files (1)
ISSN of the journal0165-5728

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