Scientific Article
previous document  unige:11619  next document
add to browser collection

Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes

Villiger, Y.
Jaconi, S.
Buisson, B.
Published in Journal of neuroimmunology. 2002, vol. 126, no. 1-2, p. 86-98
Abstract We examined the potential expression and function of alpha7 nicotinic acetylcholine receptors (nAChRs) in leukocytes. RT-PCR with alpha7 specific primers revealed the presence of the receptor mRNA in leukocytes. Immunoblotting and immunofluorescence experiments demonstrated the expression of a protein that is recognized by alpha7 specific antibodies. However, nicotine and acetylcholine (ACh) failed to elicit current in leukocytes. Binding experiments with alpha-bungarotoxin rhodamine conjugated were negative, illustrating the absence of a high-affinity binding site. RT-PCR analysis revealed the selective expression of the dupalpha7 mRNA. These data indicate that leukocytes express in their membrane the dupalpha7 protein but its physiological role remains to be identified.
Keywords Antibody SpecificityBlotting, WesternBungarotoxins/metabolism/pharmacologyCalcium/metabolismCytosol/metabolismElectrophysiologyFluorescent DyesGene Expression/immunologyHL-60 CellsHumansLeukocytes/ chemistry/ physiologyMembrane Potentials/physiologyRNA, Messenger/analysisReceptors, Nicotinic/ analysis/ genetics/immunologyRhodamines
PMID: 12020960
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
VILLIGER, Y. et al. Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes. In: Journal of neuroimmunology, 2002, vol. 126, n° 1-2, p. 86-98.

434 hits

0 download


Deposited on : 2010-08-27

Export document
Format :
Citation style :