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Scientific article
English

RFX1 is identical to enhancer factor C and functions as a transactivator of the hepatitis B virus enhancer

Published inMolecular and cellular biology, vol. 13, no. 10, p. 6375-6384
Publication date1993
Abstract

Hepatitis B virus gene expression is to a large extent under the control of enhancer I (EnhI). The activity of EnhI is strictly dependent on the enhancer factor C (EF-C) site, an inverted repeat that is bound by a ubiquitous nuclear protein known as EF-C. Here we report the unexpected finding that EF-C is in fact identical to RFX1, a novel transcription factor previously cloned by virtue of its affinity for the HLA class II X-box promoter element. This finding has allowed us to provide direct evidence that RFX1 (EF-C) is crucial for EnhI function in HepG2 hepatoma cells; RFX1-specific antisense oligonucleotides appear to inhibit EnhI-driven expression of the hepatitis B virus major surface antigen gene, and in transfection assays, RFX1 behaves as a potent transactivator of EnhI. Interestingly, transactivation of EnhI by RFX1 (EF-C) is not observed in cell lines that are not of liver origin, suggesting that the ubiquitous RFX1 protein cooperates with liver-specific factors.

Keywords
  • Animals
  • Base Sequence
  • Binding Sites
  • DNA, Viral
  • DNA-Binding Proteins/genetics/ metabolism
  • Enhancer Elements, Genetic
  • Hepatitis B virus/ genetics
  • Humans
  • Liver/metabolism
  • Mice
  • Molecular Sequence Data
  • Oligonucleotides, Antisense/pharmacology
  • Organ Specificity/genetics
  • Repetitive Sequences, Nucleic Acid
  • Trans-Activators/genetics/ metabolism
  • Transcription Factors/genetics/ metabolism
  • Viral Envelope Proteins/metabolism
Citation (ISO format)
SIEGRIST, Claire-Anne et al. RFX1 is identical to enhancer factor C and functions as a transactivator of the hepatitis B virus enhancer. In: Molecular and cellular biology, 1993, vol. 13, n° 10, p. 6375–6384.
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ISSN of the journal0270-7306
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