Motivation: Recent advances in transcriptomics have enabled unprecedented insight into gene expression analysis at a single-cell resolution. While it is anticipated that the number of publications based on such technologies will increase in the next decade, there is currently no public resource to centralize and enable scientists to explore single-cell datasets published in the field of reproductive biology. Results: Here, we present a major update of the ReproGenomics Viewer (RGV), a cross-species and cross-technology web-based resource of manually-curated sequencing datasets related to reproduction. The redesign of RGV's architecture is accompanied by significant growth of the database content including several landmark single-cell RNA sequencing datasets. The implementation of additional tools enables users to visualize and browse the complex, high-dimensional data now being generated in the reproductive field.