• Home
  1. Home
  2. Function of major histocompatibility complex class II promoters requires cooperative binding between factors RFX and NF-Y
Scientific article
English

Function of major histocompatibility complex class II promoters requires cooperative binding between factors RFX and NF-Y

Contributors

Reith, Walter; Siegrist, Claire-Anne; Durand, Bénédicte; Barras, Emmanuele; Mach, Bernard

Published in

Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 2, p. 554-558

Publication date

1994

Abstract

Transcription of major histocompatibility complex (MHC) class II genes is controlled largely by the conserved promoter elements called the X and Y boxes. We show here that RFX, the X box-binding protein deficient in certain MHC class II-deficient immunodeficiency patients (CID), and the Y box-binding protein NF-Y bind cooperatively. Functional relevance of this protein-protein interaction is suggested by the fact that promoter activity correlates with cooperative binding of RFX and NF-Y rather than with binding of RFX or NF-Y alone. Stability of the RFX/NF-Y complex is affected by alterations in X-Y box spacing. These results are consistent with the fact that MHC class II promoter function is dependent on correct stereospecific alignment of the X and Y boxes. Cooperative binding involving RFX, NF-Y, and perhaps other MHC class II promoter-binding proteins may explain why the highly specific defect in binding of RFX observed in CID cells is associated in vivo with a bare promoter in which all of the cis-acting elements, including the X and Y boxes, are unoccupied.

Keywords

  • Base Sequence
  • Binding Sites
  • CCAAT-Enhancer-Binding Proteins
  • Cell Line
  • DNA/genetics/metabolism
  • DNA-Binding Proteins/ metabolism
  • Genes, MHC Class II
  • Humans
  • Immunologic Deficiency Syndromes/genetics/immunology/metabolism
  • Molecular Sequence Data
  • Phenotype
  • Promoter Regions, Genetic
  • Transcription Factors/ metabolism
  • Transcription, Genetic

UNIGE affiliation entities

Citation (ISO format)

REITH, Walter et al. Function of major histocompatibility complex class II promoters requires cooperative binding between factors RFX and NF-Y. In: Proceedings of the National Academy of Sciences of the United States of America, 1994, vol. 91, n° 2, p. 554–558. doi: 10.1073/pnas.91.2.554

Main files (1)

Article

Identifiers

Additional URL for this publication

http://www.pnas.org/content/91/2/554.full.pdf

Journal ISSN

0027-8424
618views
0downloads

Technical informations

Creation

27/08/2010 13:35:57

First validation

27/08/2010 13:35:57

Update

14/03/2023 16:05:01

Status update

14/03/2023 16:05:01

Last indexation

29/10/2024 17:01:11
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated