en
Scientific article
English

Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors

Published inFEBS letters, vol. 487, no. 3, p. 377-383
Publication date2001
Abstract

The molecular basis of capacitative (or store-operated) Ca2+ entry is still subject to debate. The transient receptor potential proteins have been hypothesized to be structural components of store-operated Ca2+ channels and recent evidence suggests that Trp3 and its closely related homolog Trp6 are gated by the N-terminal region of the inositol 1,4,5-triphosphate receptors (InsP3R). In this study, we report the existence of two isoforms of the human Trp4 protein, referred to as alpha-hTrp4 and beta-hTrp4. The shorter variant beta-hTrp4 is generated through alternative splicing and lacks the C-terminal amino acids G785-S868. Using a yeast two-hybrid assay and glutathione-S-transferase-pulldown experiments, we found that the C-terminus of alpha-hTrp4, but not of beta-hTrp4, associates in vitro with the C-terminal domain of the InsP(3) receptors type 1, 2 and 3. Thus, we describe a novel interaction between Trp proteins and InsP3R and we provide evidence suggesting that the formation of hTrp4-InsP3R complexes may be regulated by alternative splicing.

Keywords
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Calcium Channels/ chemistry/classification/ genetics/ metabolism
  • DNA Primers/genetics
  • Humans
  • Inositol 1,4,5-Trisphosphate Receptors
  • Molecular Sequence Data
  • Protein Isoforms/chemistry/genetics/metabolism
  • RNA, Messenger/genetics/metabolism
  • Rats
  • Receptors, Cytoplasmic and Nuclear/ chemistry/classification/genetics/ metabolism
  • Sequence Homology, Amino Acid
  • TRPC Cation Channels
  • Tissue Distribution
  • Two-Hybrid System Techniques
Affiliation Not a UNIGE publication
Citation (ISO format)
MERY, Laurence et al. Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors. In: FEBS letters, 2001, vol. 487, n° 3, p. 377–383. doi: 10.1016/s0014-5793(00)02362-0
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ISSN of the journal0014-5793
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