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Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors
|Published in||FEBS Letters. 2001, vol. 487, no. 3, p. 377-383|
|Abstract||The molecular basis of capacitative (or store-operated) Ca2+ entry is still subject to debate. The transient receptor potential proteins have been hypothesized to be structural components of store-operated Ca2+ channels and recent evidence suggests that Trp3 and its closely related homolog Trp6 are gated by the N-terminal region of the inositol 1,4,5-triphosphate receptors (InsP3R). In this study, we report the existence of two isoforms of the human Trp4 protein, referred to as alpha-hTrp4 and beta-hTrp4. The shorter variant beta-hTrp4 is generated through alternative splicing and lacks the C-terminal amino acids G785-S868. Using a yeast two-hybrid assay and glutathione-S-transferase-pulldown experiments, we found that the C-terminus of alpha-hTrp4, but not of beta-hTrp4, associates in vitro with the C-terminal domain of the InsP(3) receptors type 1, 2 and 3. Thus, we describe a novel interaction between Trp proteins and InsP3R and we provide evidence suggesting that the formation of hTrp4-InsP3R complexes may be regulated by alternative splicing.|
|Keywords||Alternative Splicing — Amino Acid Sequence — Animals — Base Sequence — Binding Sites — Calcium Channels/ chemistry/classification/ genetics/ metabolism — DNA Primers/genetics — Humans — Inositol 1,4,5-Trisphosphate Receptors — Molecular Sequence Data — Protein Isoforms/chemistry/genetics/metabolism — RNA, Messenger/genetics/metabolism — Rats — Receptors, Cytoplasmic and Nuclear/ chemistry/classification/genetics/ metabolism — Sequence Homology, Amino Acid — TRPC Cation Channels — Tissue Distribution — Two-Hybrid System Techniques|
|MERY, Laurence et al. Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors. In: FEBS Letters, 2001, vol. 487, n° 3, p. 377-383. doi: 10.1016/s0014-5793(00)02362-0 https://archive-ouverte.unige.ch/unige:11468|