Scientific article

Promoter-specific functions of CIITA and the MHC class II enhanceosome in transcriptional activation

Published inEMBO journal, vol. 21, no. 6, p. 1379-1388
Publication date2002

Transcription of the major histocompatibility complex class II family of genes is regulated by conserved promoter elements and two gene-specific trans-activators, RFX and CIITA. RFX binds DNA and nucleates the assembly of an enhanceosome, which recruits CIITA through protein--protein interactions. Transcriptional activation is a complex, multi-step process involving chromatin modification and recruitment of the transcription apparatus. To examine the roles of the enhanceosome and CIITA in these processes, we analysed the level of promoter-associated hyperacetylated histones H3 and H4, TBP, TFIIB and RNA poly merase II in cells lacking RFX or CIITA. We compared four genes co-regulated by RFX and CIITA (HLA-DRA, HLA-DPB, HLA-DMB and Ii) and found that the enhanceosome and CIITA make variable, promoter-dependent contributions to histone acetylation and transcription apparatus recruitment. CIITA is generally implicated at multiple levels of the activation process, while the enhanceosome contributes in a CIITA-independent manner only at certain promoters. Our results support the general notion that the impact of a particular activator on transcription in vivo may vary depending on the promoter and the chromatin context.

  • Acetylation
  • Acetyltransferases/metabolism
  • Antigens, Differentiation, B-Lymphocyte/ genetics
  • B-Lymphocytes/cytology
  • Cell Line
  • DNA-Binding Proteins/metabolism
  • Enhancer Elements, Genetic
  • Genes, MHC Class II
  • HLA-D Antigens/ genetics
  • HLA-DP Antigens/genetics
  • HLA-DR Antigens/genetics
  • Histocompatibility Antigens Class II/ genetics
  • Histone Acetyltransferases
  • Histones/metabolism
  • Humans
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators/genetics/ metabolism
  • Transcription Factors/metabolism
  • Transcriptional Activation
Affiliation Not a UNIGE publication
Citation (ISO format)
MASTERNAK, Krzysztof, REITH, Walter. Promoter-specific functions of CIITA and the MHC class II enhanceosome in transcriptional activation. In: EMBO journal, 2002, vol. 21, n° 6, p. 1379–1388. doi: 10.1093/emboj/21.6.1379
Main files (1)
ISSN of the journal0261-4189

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