Scientific Article
previous document  unige:11418  next document
add to browser collection

Nucleosome eviction from MHC class II promoters controls positioning of the transcription start site

Seguin-Estevez, Queralt
Schmid, C. D.
Ambrosini, Giovanna
Bucher, Philipp
Published in Nucleic Acids Research. 2009, vol. 37, no. 8, p. 2514-2528
Abstract Nucleosome depletion at transcription start sites (TSS) has been documented genome-wide in multiple eukaryotic organisms. However, the mechanisms that mediate this nucleosome depletion and its functional impact on transcription remain largely unknown. We have studied these issues at human MHC class II (MHCII) genes. Activation-induced nucleosome free regions (NFR) encompassing the TSS were observed at all MHCII genes. Nucleosome depletion was exceptionally strong, attaining over 250-fold, at the promoter of the prototypical HLA-DRA gene. The NFR was induced primarily by the transcription factor complex that assembles on the conserved promoter-proximal enhancer situated upstream of the TSS. Functional analyses performed in the context of native chromatin demonstrated that displacing the NFR without altering the sequence of the core promoter induced a shift in the position of the TSS. The NFR thus appears to play a critical role in transcription initiation because it directs correct TSS positioning in vivo. Our results provide support for a novel mechanism in transcription initiation whereby the position of the TSS is controlled by nucleosome eviction rather than by promoter sequence.
Keywords B-Lymphocytes/immunologyCell Line, TumorChromatin Assembly and DisassemblyGenes, MHC Class IIHLA-DR Antigens/geneticsHumansInterferon-gamma/pharmacologyNucleosomes/ metabolismPromoter Regions, GeneticTranscription Initiation Site
PMID: 19264803
Full text
(ISO format)
LEIMGRUBER, Elisa et al. Nucleosome eviction from MHC class II promoters controls positioning of the transcription start site. In: Nucleic Acids Research, 2009, vol. 37, n° 8, p. 2514-2528.

307 hits

0 download


Deposited on : 2010-08-27

Export document
Format :
Citation style :