Scientific Article
previous document  unige:113716  next document
add to browser collection

Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche

Chow, Andrew
Lucas, Daniel
Hidalgo, Andrés
Méndez-Ferrer, Simón
Hashimoto, Daigo
Battista, Michela
Leboeuf, Marylene
show hidden authors show all authors [1 - 13]
Published in Journal of Experimental Medicine. 2011, vol. 208, no. 2, p. 261-271
Abstract Hematopoietic stem cells (HSCs) reside in specialized bone marrow (BM) niches regulated by the sympathetic nervous system (SNS). Here, we have examined whether mononuclear phagocytes modulate the HSC niche. We defined three populations of BM mononuclear phagocytes that include Gr-1(hi) monocytes (MOs), Gr-1(lo) MOs, and macrophages (MΦ) based on differential expression of Gr-1, CD115, F4/80, and CD169. Using MO and MΦ conditional depletion models, we found that reductions in BM mononuclear phagocytes led to reduced BM CXCL12 levels, the selective down-regulation of HSC retention genes in Nestin(+) niche cells, and egress of HSCs/progenitors to the bloodstream. Furthermore, specific depletion of CD169(+) MΦ, which spares BM MOs, was sufficient to induce HSC/progenitor egress. MΦ depletion also enhanced mobilization induced by a CXCR4 antagonist or granulocyte colony-stimulating factor. These results highlight two antagonistic, tightly balanced pathways that regulate maintenance of HSCs/progenitors in the niche during homeostasis, in which MΦ cross talk with the Nestin(+) niche cell promotes retention, and in contrast, SNS signals enhance egress. Thus, strategies that target BM MΦ hold the potential to augment stem cell yields in patients that mobilize HSCs/progenitors poorly.
Keywords AnimalsBone Marrow Cells/cytology/metabolismChemokine CXCL12/metabolismColony-Forming Units AssayDNA Primers/geneticsEnzyme-Linked Immunosorbent AssayFlow CytometryFluorescent Antibody TechniqueHematopoietic Stem Cell MobilizationHematopoietic Stem Cells/cytologyHomeostasis/immunologyInterleukin-10/geneticsMacrophages/metabolismMaleMembrane Glycoproteins/metabolismMesenchymal Stromal Cells/cytologyMiceMice, Inbred C57BLMice, KnockoutMonocytes/metabolismPolymerase Chain ReactionReceptor Cross-Talk/immunologyReceptors, Immunologic/metabolismSialic Acid Binding Ig-like Lectin 1
PMID: 21282381
Full text
Article (Published version) (2.7 MB) - public document Free access
Research group L'influence du rôle du cycle circadien sur l'immunité (995)
(ISO format)
CHOW, Andrew et al. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. In: Journal of Experimental Medicine, 2011, vol. 208, n° 2, p. 261-271. doi: 10.1084/jem.20101688

264 hits



Deposited on : 2019-01-31

Export document
Format :
Citation style :