Scientific Article
previous document  unige:11368  next document
add to browser collection

Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia

Kamiguti, A. S.
Lin, Ke
Harris, R. J.
Cawley, J. C.
Allsup, D. J.
Slupsky, J. R.
show hidden authors show all authors [1 - 9]
Published in Journal of Immunology. 2005, vol. 175, no. 12, p. 8424-8430
Abstract Hairy cells (HCs) are mature malignant B cells that contain a number of constitutively active signaling molecules including GTP-bound Rac1, protein kinase C, and Src family kinases. Because Rac1 is a component of the reactive oxidant species (ROS)-generating NADPH oxidase system, we investigated the role of this GTPase in ROS production in HCs. In this study, we show that ROS production in HCs involves a flavin-containing oxidase dependent on Ca2+, but not on GTP-Rac1 or protein kinase C. This suggests the involvement of the nonphagocytic NADPH oxidase NOX5, an enzyme found in lymphoid tissues, but not in circulating lymphocytes. By using RT-PCR and Southern and Western blotting and by measuring superoxide anion production in membrane fractions in the absence of cytosolic components, we demonstrate for the first time that HCs (but not circulating normal B cells or some other lymphoid cell types) express NOX5. We also demonstrate that inhibition of NADPH oxidase in HCs results in a selective increase in the activity of Src homology region 2 domain-containing phosphatase 1 (SHP-1). Furthermore, SHP-1 in HCs coimmunoprecipitates with tyrosine phosphorylated CD22 and localizes in the same cellular compartment as NOX5. This allows the inactivation of SHP-1 by NOX5-generated ROS and contributes to the maintenance of the constitutive activation of HCs.
Keywords Antigens, CD22/metabolismB-Lymphocytes/ enzymologyCalciumCell FractionationHumansIntracellular Signaling Peptides and Proteins/metabolismLeukemia, Hairy Cell/ pathologyMembrane Proteins/analysis/ metabolismNADPH Oxidase/analysis/ metabolismNeoplastic Cells, Circulating/ chemistryProtein Phosphatase 1Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine Phosphatases/metabolismReactive Oxygen Species/analysis/metabolismSuperoxides/analysis/metabolismrac1 GTP-Binding Protein
PMID: 16339585
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version:
(ISO format)
KAMIGUTI, A. S. et al. Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia. In: Journal of Immunology, 2005, vol. 175, n° 12, p. 8424-8430. doi: 10.4049/jimmunol.175.12.8424

356 hits

0 download


Deposited on : 2010-08-27

Export document
Format :
Citation style :