en
Scientific article
English

Inherited immunodeficiency with a defect in a major histocompatibility complex class II promoter-binding protein differs in the chromatin structure of the HLA-DRA gene

Published inMolecular and cellular biology, vol. 9, no. 1, p. 296-302
Publication date1989
Abstract

A defect in a trans-regulatory factor which controls major histocompatibility complex class II gene expression is responsible for an inherited form of immunodeficiency with a lack of expression of human leukocyte antigen (HLA) class II antigens. We have recently described and cloned an HLA class II promoter DNA-binding protein, RF-X, present in normal B cells and absent in these class II-deficient regulatory mutants. Here we report that these in vitro results correlate with a specific change in the chromatin structure of the class II promoter: two prominent DNase I-hypersensitive sites were identified in the promoter of the HLA-DRA gene in normal B lymphocytes and found to be absent in the class II-deficient mutant cells. The same two prominent DNase I-hypersensitive sites were observed in normal fibroblastic cells induced by gamma interferon to express class II genes. Interestingly, they were also observed in the uninduced class II-negative fibroblastic cells, which have also been shown to have a normal RF-X binding pattern. We conclude that the two DNase I-hypersensitive sites in the HLA-DRA promoter reflect features in chromatin structure which correlate with the binding of the trans-acting factor RF-X and which are necessary but not sufficient for the expression of class II genes.

Keywords
  • B-Lymphocytes/metabolism
  • Blotting, Southern
  • Cell Line, Transformed
  • Chromatin/ analysis
  • DNA-Binding Proteins/ genetics
  • Deoxyribonuclease I/diagnostic use
  • Genes, MHC Class II
  • HLA-D Antigens/ genetics
  • Herpesvirus 4, Human/metabolism
  • Histocompatibility Antigens Class II/ genetics
  • Humans
  • Immunologic Deficiency Syndromes/ genetics
  • Mutation
  • Promoter Regions, Genetic
  • RNA/analysis
Citation (ISO format)
GÖNCZY, P. et al. Inherited immunodeficiency with a defect in a major histocompatibility complex class II promoter-binding protein differs in the chromatin structure of the HLA-DRA gene. In: Molecular and cellular biology, 1989, vol. 9, n° 1, p. 296–302. doi: 10.1128/mcb.9.1.296
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0270-7306
559views
0downloads

Technical informations

Creation08/27/2010 1:34:37 PM
First validation08/27/2010 1:34:37 PM
Update time03/14/2023 4:03:25 PM
Status update03/14/2023 4:03:25 PM
Last indexation02/12/2024 7:17:24 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack