UNIGE document Scientific Article
previous document  unige:112587  next document
add to browser collection

Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation

Published in Chemistry - A European Journal. 2019, vol. 25, no. 1, p. 334-342
Abstract Here, the synthesis of a RuII photocatalyst by light‐directed oligonucleotide‐templated ligation reaction is described. The photocatalyst was found to have tremendous potential for signal amplification with >15000 turnovers measured in 9 hours. A templated reaction was used to turn on the activity of this ruthenium(II) photocatalyst in response to a specific DNA sequence. The photocatalysis of the ruthenium(II) complex was harnessed to uncage a new precipitating dye that is highly fluorescent and photostable in the solid state. This reaction was used to discriminate between different DNA analytes based on localization of the precipitate as well as for in cellulo miRNA detection. Finally, a bipyridine ligand functionalized with two different peptide nucleic acid (PNA) sequences was shown to enable template‐mediated ligation (turn on of the ruthenium(II) photocatalysis) and recruitment of substrate for templated photocatalysis.
Full text
Article (Published version) (1.8 MB) - document accessible for UNIGE members only Limited access to UNIGE
Article (Accepted version) (1.3 MB) - public document Free access
Research group Groupe Winssinger
(ISO format)
ANZOLA, Marcello, WINSSINGER, Nicolas. Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation. In: Chemistry - A European Journal, 2019, vol. 25, n° 1, p. 334-342. doi: 10.1002/chem.201804283 https://archive-ouverte.unige.ch/unige:112587

261 hits



Deposited on : 2019-01-08

Export document
Format :
Citation style :