• Home
  1. Home
  2. Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation
Scientific article
OA Policy
English

Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation

Contributors

Anzola, Marcello; Winssinger, Nicolas

Published in

Chemistry - A European Journal, vol. 25, no. 1, p. 334-342

Publication date

2019

Abstract

Here, the synthesis of a RuII photocatalyst by light‐directed oligonucleotide‐templated ligation reaction is described. The photocatalyst was found to have tremendous potential for signal amplification with >15000 turnovers measured in 9 hours. A templated reaction was used to turn on the activity of this ruthenium(II) photocatalyst in response to a specific DNA sequence. The photocatalysis of the ruthenium(II) complex was harnessed to uncage a new precipitating dye that is highly fluorescent and photostable in the solid state. This reaction was used to discriminate between different DNA analytes based on localization of the precipitate as well as for in cellulo miRNA detection. Finally, a bipyridine ligand functionalized with two different peptide nucleic acid (PNA) sequences was shown to enable template‐mediated ligation (turn on of the ruthenium(II) photocatalysis) and recruitment of substrate for templated photocatalysis.

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

ANZOLA, Marcello, WINSSINGER, Nicolas. Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation. In: Chemistry - A European Journal, 2019, vol. 25, n° 1, p. 334–342. doi: 10.1002/chem.201804283

Main files (2)

Article (Published version)
Article (Accepted version)

Identifiers

Journal ISSN

0947-6539
608views
693downloads

Technical informations

Creation

07/01/2019 17:29:00

First validation

07/01/2019 17:29:00

Update

13/10/2025 18:36:12

Status update

15/03/2023 15:20:14

Last indexation

01/07/2026 06:11:17
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated