Scientific article

Pax6-induced alteration of cell fate: shape changes, expression of neuronal alpha tubulin, postmitotic phenotype, and cell migration

Published inJournal of neurobiology, vol. 66, no. 5, p. 421-436
Publication date2006

The transcription factor Pax6 plays an important role in the development of the central nervous system. To understand its mechanism of action, we transduced HeLa cells with a Pax6-expressing lentiviral vector. Upon transduction, HeLa cells markedly changed shape and formed neuritelike extensions. Pax6-transduced HeLa cells expressed high levels of neuronal alpha3 tubulin, demonstrating a partial transdifferentiation towards a neuronal phenotype. Neurons are postmitotic cells. Pax6-transduced HeLa cells became postmitotic through mechanisms involving up-regulation of p53 and cyclin-dependent kinase inhibitor p21. One of the most striking effects of Pax6 was observed by time-lapse videomicroscopy: cells started to dissociate from cell clusters and displayed intense migratory activity. Migration was accompanied by dynamic and reversible shape changes. Our results identified three elements of Pax6 action: (i) expression of neuron-specific genes; (ii) establishment of a postmitotic phenotype; and (iii) involvement in the regulation of cell shape and cell migration.

  • Animals
  • Cell Cycle/physiology
  • Cell Differentiation/ physiology
  • Cell Division/physiology
  • Cell Lineage/physiology
  • Cell Movement/ physiology
  • Cell Shape/physiology
  • Cells, Cultured
  • Central Nervous System/cytology/ embryology/metabolism
  • Cyclin-Dependent Kinase Inhibitor p21/metabolism
  • Eye Proteins/genetics/ metabolism
  • Hela Cells
  • Homeodomain Proteins/genetics/ metabolism
  • Humans
  • Mice
  • Neurites/metabolism/ultrastructure
  • Neurons/cytology/ metabolism
  • Paired Box Transcription Factors/genetics/ metabolism
  • Phenotype
  • Repressor Proteins/genetics/ metabolism
  • Transduction, Genetic
  • Tubulin/ metabolism
  • Tumor Suppressor Protein p53/metabolism
Citation (ISO format)
CARTIER, Laetitia et al. Pax6-induced alteration of cell fate: shape changes, expression of neuronal alpha tubulin, postmitotic phenotype, and cell migration. In: Journal of neurobiology, 2006, vol. 66, n° 5, p. 421–436. doi: 10.1002/neu.20225
Updates (1)
ISSN of the journal0022-3034

Technical informations

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