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Venular basement membranes contain specific matrix protein low expression regions that act as exit points for emigrating neutrophils

Wang, Shijun
Voisin, Mathieu-Benoit
Larbi, Karen Y
Dangerfield, John
Tran, Maxine
Maxwell, Patrick H
Sorokin, Lydia
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Published in Journal of Experimental Medicine. 2006, vol. 203, no. 6, p. 1519-1532
Abstract The mechanism of leukocyte migration through venular walls in vivo is largely unknown. By using immunofluorescence staining and confocal microscopy, the present study demonstrates the existence of regions within the walls of unstimulated murine cremasteric venules where expression of key vascular basement membrane (BM) constituents, laminin 10, collagen IV, and nidogen-2 (but not perlecan) are considerably lower (<60%) than the average expression detected in the same vessel. These sites were closely associated with gaps between pericytes and were preferentially used by migrating neutrophils during their passage through cytokine-stimulated venules. Although neutrophil transmigration did not alter the number/unit area of extracellular matrix protein low expression sites, the size of these regions was enlarged and their protein content was reduced in interleukin-1beta-stimulated venules. These effects were entirely dependent on the presence of neutrophils and appeared to involve neutrophil-derived serine proteases. Furthermore, evidence was obtained indicating that transmigrating neutrophils carry laminins on their cell surface in vivo. Collectively, through identification of regions of low extracellular matrix protein localization that define the preferred route for transmigrating neutrophils, we have identified a plausible mechanism by which neutrophils penetrate the vascular BM without causing a gross disruption to its intricate structure.
Keywords AnimalsBasement Membrane/physiologyCell MovementDNA PrimersExtracellular Matrix Proteins/geneticsGene Expression RegulationLeukocytes/physiologyMiceMice, Inbred C57BLMuscle, Skeletal/blood supplyNeutrophils/physiologyReverse Transcriptase Polymerase Chain ReactionVenules/physiology
PMID: 16754715
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Research group Groupe Christoph Scheiermann (995)
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WANG, Shijun et al. Venular basement membranes contain specific matrix protein low expression regions that act as exit points for emigrating neutrophils. In: Journal of Experimental Medicine, 2006, vol. 203, n° 6, p. 1519-1532. doi: 10.1084/jem.20051210

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Deposited on : 2018-11-28

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