Heterogeneity in the progeny of single human muscle satellite cells

Baroffio Barbier, Anne; Bochaton-Piallat, Marie-Luce; Gabbiani, Giulio; Bader, C. R.

doi:10.1046/j.1432-0436.1995.5940259.x

Advanced search

Browse by...

Deposit

Highlights

More informations

Title		Heterogeneity in the progeny of single human muscle satellite cells
Authors		Baroffio Barbier, Anne Bochaton-Piallat, Marie-Luce Gabbiani, Giulio Bader, C. R.
Published in		Differentiation. 1995, vol. 59, no. 4, p. 259-268
Abstract		We examined whether freshly isolated (native) human muscle satellite cells (HMSC), as well as their proliferating clonal progenies, were heterogeneous. We studied the expression of the cytoskeletal proteins, desmin (DSM), alpha-sarcomeric and alpha-smooth muscle actins (alpha-SR actin, alpha-SM actin), three markers that may be expressed prior to the fusion process. We found that native HMSC constituted a homogeneous population of cells expressing desmin and giving rise to similar clones in vitro. The clonal progeny of HMSC was heterogeneous, including several subpopulations of myoblasts with different cytoskeletal phenotypes, commitment states and fusion abilities. A major subpopulation that expressed both alpha-sarcomeric actin and desmin during the proliferative stage corresponded to a "predifferentiated" population of myoblasts, committed to fusion. Another subpopulation, expressing exclusively desmin, and phenotypically similar to native HMSC, failed to fuse under fusion-promoting conditions and could represent a new generation of HMSC born in culture.
Keywords		Actins/ analysis — Adolescent — Adult — Cell Division/physiology — Child — Child, Preschool — Clone Cells — Cytoskeletal Proteins/ analysis — Desmin/ analysis — Genetic Heterogeneity — Humans — Infant — Muscle Proteins/ analysis — Muscle, Skeletal/cytology/ metabolism — Phenotype
Identifiers		DOI: 10.1046/j.1432-0436.1995.5940259.x PMID: 8575648
Full text		Article (Published version) - Limited access to UNIGE Other version: https://www.sciencedirect.com/science/article/pii/S0301468109608136
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Citation (ISO format)		BAROFFIO BARBIER, Anne et al. Heterogeneity in the progeny of single human muscle satellite cells. In: Differentiation, 1995, vol. 59, n° 4, p. 259-268. doi: 10.1046/j.1432-0436.1995.5940259.x https://archive-ouverte.unige.ch/unige:11136