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Scientific article
English

Induction of HLA class II genes by IFN-gamma is transcriptional and requires a trans-acting protein

Published inThe Journal of immunology, vol. 142, no. 3, p. 999-1004
Publication date1989
Abstract

HLA class II Ag are encoded by a family of related genes clustered in the HLA-D region of the MHC. The expression of this multi-gene family is highly regulated and this regulation is essential for the control of the immune response. Class II gene expression is constitutive in a limited number of cell types and can be induced by IFN-gamma in a number of class II negative cells. In this study, we have clarified two essential aspects of the regulation of HLA class II genes by IFN-gamma. 1) The induction mechanism operates at the level of transcription and there is a long lag phase in the signal transduction process. 2) The induction of class II genes requires the de novo synthesis of a new protein(s). On this basis, we propose that IFN-gamma regulates the transcription of HLA class II genes via the de novo synthesis of a trans-acting activator protein.

Keywords
  • Antigens, Differentiation, B-Lymphocyte
  • Cell Line
  • Gene Expression Regulation/ drug effects
  • Genes, MHC Class II/ drug effects
  • HLA-DR Antigens/ genetics
  • Histocompatibility Antigens Class II/genetics
  • Humans
  • Interferon-gamma/ pharmacology
  • Kinetics
  • Osteosarcoma/genetics
  • Protein Biosynthesis
  • Transcription Factors/ pharmacology
  • Transcription, Genetic/ drug effects
Citation (ISO format)
AMALDI, I. et al. Induction of HLA class II genes by IFN-gamma is transcriptional and requires a trans-acting protein. In: The Journal of immunology, 1989, vol. 142, n° 3, p. 999–1004.
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ISSN of the journal0022-1767
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