UNIGE document Doctoral Thesis
previous document  unige:110627  next document
add to browser collection
Title

Identification and analysis of the putative AMP-activated protein kinase (AMPK) in blood stream form of Trypanosoma brucei

Author
Directors
Defense Thèse de doctorat : Univ. Genève, 2018 - Sc. 5239 - 2018/06/28
Abstract Human African trypanosomiasis caused by the infection of Trypanosoma brucei (T. brucei) is a fatal disease if untreated. Today treatments are either very toxic, very complicated to administer or limited to the first phase of the infection, thus new potent and safe drugs are needed. The aim of the thesis was to investigate the AMP-activated protein kinase (AMPK) in T. brucei. AMPK is a heterotrimeric complex known for its role in maintaining energy homeostasis in the cell by detecting the ratio AMP:ATP. To this end the corresponding subunits were identified by sequence alignment and confirmed by a modified bacterial adenylate two-hybrid system : catalytic subunits α (TbAMPKα1: Tb927.3.4560, TbAMPKα2: Tb927.10.5310) and regulatory subunits β (Tb927.8.2450) and γ (Tb927.10.3700). The complex was expressed using a polycistronic expression system and the purified complex was biochemically characterized. Finally, the importance of the catalytic subunits were confirmed in vivo on designed knock-out and knock-down strains.
Identifiers
URN: urn:nbn:ch:unige-1106277
Full text
Thesis (19.2 MB) - private document Private access (until 2019-11-11)
Structures
Research group Biochimie pharmaceutique
Project FNS: 31003A_140722/1 S17782
Citation
(ISO format)
AMBUEHL, Chiara. Identification and analysis of the putative AMP-activated protein kinase (AMPK) in blood stream form of Trypanosoma brucei. Université de Genève. Thèse, 2018. https://archive-ouverte.unige.ch/unige:110627

46 hits

1 download

Update

Deposited on : 2018-11-12

Export document
Format :
Citation style :