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PATL2 is a key actor of oocyte maturation whose invalidation causes infertility in women and mice

Christou-Kent, Marie
Kherraf, Zine-Eddine
Amiri-Yekta, Amir
Le Blévec, Emilie
Karaouzène, Thomas
Assou, Said
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Published in EMBO Molecular Medicine. 2018, vol. 10, no. 5, p. 459-66
Abstract The genetic causes of oocyte meiotic deficiency (OMD), a form of primary infertility characterised by the production of immature oocytes, remain largely unexplored. Using whole exome sequencing, we found that 26% of a cohort of 23 subjects with OMD harboured the same homozygous nonsense pathogenic mutation in PATL2, a gene encoding a putative RNA-binding protein. Using Patl2 knockout mice, we confirmed that PATL2 deficiency disturbs oocyte maturation, since oocytes and zygotes exhibit morphological and developmental defects, respectively. PATL2's amphibian orthologue is involved in the regulation of oocyte mRNA as a partner of CPEB However, Patl2's expression profile throughout oocyte development in mice, alongside colocalisation experiments with Cpeb1, Msy2 and Ddx6 (three oocyte RNA regulators) suggest an original role for Patl2 in mammals. Accordingly, transcriptomic analysis of oocytes from WT and Patl2-/- animals demonstrated that in the absence of Patl2, expression levels of a select number of highly relevant genes involved in oocyte maturation and early embryonic development are deregulated. In conclusion, PATL2 is a novel actor of mammalian oocyte maturation whose invalidation causes OMD in humans.
Keywords AnimalsColumbidaeElectron Probe MicroanalysisEnvironmental Exposure/adverse effectsEpithelium/drug effects/pathologyFungicidesIndustrial/adverse effectsLung/drug effects/ultrastructureManeb/adverse effectsMicroscopyElectronScanningModelsBiologicalSentinel SurveillanceTrachea/drug effects/ultrastructureZineb/adverse effects
PMID: 29661911
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Research group Mécanismes moléculaires et génétiques du développement sexuel (778)
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CHRISTOU-KENT, Marie et al. PATL2 is a key actor of oocyte maturation whose invalidation causes infertility in women and mice. In: EMBO Molecular Medicine, 2018, vol. 10, n° 5, p. 459-66. doi: 10.15252/emmm.201708515 https://archive-ouverte.unige.ch/unige:109588

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Deposited on : 2018-10-23

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