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Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2

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Published in Nature Cell Biology. 2018, vol. 20, no. 9, p. 1043-1051
Abstract The target of rapamycin complex 2 (TORC2) plays a key role in maintaining the homeostasis of plasma membrane (PM) tension. TORC2 activation following increased PM tension involves redistribution of the Slm1 and 2 paralogues from PM invaginations known as eisosomes into membrane compartments containing TORC2. How Slm1/2 relocalization is triggered, and if/how this plays a role in TORC2 inactivation with decreased PM tension, is unknown. Using osmotic shocks and palmitoylcarnitine as orthogonal tools to manipulate PM tension, we demonstrate that decreased PM tension triggers spontaneous, energy-independent reorganization of pre-existing phosphatidylinositol-4,5-bisphosphate into discrete invaginated membrane domains, which cluster and inactivate TORC2. These results demonstrate that increased and decreased membrane tension are sensed through different mechanisms, highlighting a role for membrane lipid phase separation in mechanotransduction.
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Other version: http://www.nature.com/articles/s41556-018-0150-z
Research groups Groupe Loewith
Groupe Matile
Groupe Roux
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RIGGI, Margot et al. Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2. In: Nature Cell Biology, 2018, vol. 20, n° 9, p. 1043-1051. doi: 10.1038/s41556-018-0150-z https://archive-ouverte.unige.ch/unige:107468

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Deposited on : 2018-09-05

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