Scientific article
Review
OA Policy
English

New developments and possibilities of wide-pore superficially porous particle technology applied for the liquid chromatographic analysis of therapeutic proteins

Published inJournal of Pharmaceutical and Biomedical Analysis, vol. 158, p. 225-235
Publication date2018
Abstract

This review paper discusses the success of columns packed with superficially porous particles (SPP) in liquid chromatography for the analysis of peptides and proteins. First, it summarizes the history of SPP, including the development of different SPP generations from particles of 50 um to sub-2 um. It also critically discusses the improved kinetic performance of SPP particles in comparison to fully porous particles. The current trends and applications of columns packed with SPPs for the analysis of peptides and proteins (including mAbs and ADC at the intact and sub-unit levels) are shown, as well. Finally, some of the potential perspectives for this technology are also described, including the radially oriented mesopores or the applicability of the technology for chiral separations.

Keywords
  • Superficially porous particles
  • Core-shell
  • Wide-pore
  • Protein
  • Monoclonal antibody
  • Antibody-drug-conjugate
Research groups
Citation (ISO format)
BOBALY, Balazs et al. New developments and possibilities of wide-pore superficially porous particle technology applied for the liquid chromatographic analysis of therapeutic proteins. In: Journal of Pharmaceutical and Biomedical Analysis, 2018, vol. 158, p. 225–235. doi: 10.1016/j.jpba.2018.06.006
Main files (2)
Article (Published version)
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Article (Accepted version)
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Identifiers
Journal ISSN0731-7085
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305downloads

Technical informations

Creation06/13/2018 2:31:00 PM
First validation06/13/2018 2:31:00 PM
Update time03/15/2023 8:27:08 AM
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