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Scientific article
English

Parallel artificial membrane permeability assay: a new membrane for the fast prediction of passive human skin permeability

Published inJournal of medicinal chemistry, vol. 49, no. 13, p. 3948-3954
Publication date2006
Abstract

This work was devoted to the search for new artificial membranes allowing a rapid evaluation of passive human skin permeation of compounds with a parallel artificial membrane permeability assay (PAMPA). Effective permeability coefficients (Pe) determined for a set of compounds using the PAMPA technique with isopropyl myristate (IPM) and silicone oil, alone or in mixture, were compared to the corresponding human skin permeability coefficient values (Kp). A good correlation between Pe and Kp was found for compounds tested through a membrane consisting of 70% silicone and 30% IPM. Moreover, positive correlation between the membrane retention of compounds and stratum corneum/water partition coefficients (PSC) was established. These results showed that this new artificial membrane, defined as PAMPA-skin, is able to mimic the main barrier properties of human stratum corneum and can be used for the fast prediction of passive human skin permeability coefficients.

Keywords
  • Diffusion
  • Dimethylpolysiloxanes/*chemistry
  • Humans
  • Membranes, Artificial
  • Myristates/*chemistry
  • Permeability
  • Skin/*metabolism
  • Skin Absorption
  • Water/chemistry
Citation (ISO format)
OTTAVIANI, G., MARTEL, Sophie, CARRUPT, Pierre-Alain. Parallel artificial membrane permeability assay: a new membrane for the fast prediction of passive human skin permeability. In: Journal of medicinal chemistry, 2006, vol. 49, n° 13, p. 3948–3954. doi: 10.1021/jm060230%2B
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ISSN of the journal0022-2623
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