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A comparison of cotton and flocked swabs for vaginal self-sample collection
|Published in||International journal of women's health. 2018, vol. Volume 10, p. 229-236|
|Abstract||OBJECTIVE: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval. METHODS: We recruited women aged 21-65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt® vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample. RESULTS: A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively (p=0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (p<0.001). The mean number of cells detected at cytological cell count was 13,130.42 using the cotton swab and 17,503.6 using the flocked swab (p<0.001). CONCLUSION: The flocked swab achieved a greater cellular retrieval and showed an improved performance in HPV detection. Further studies are needed to assess the usability and cost-effectiveness of the 2 self-sampling devices.|
|Research groups||Laboratoire de biologie des tumeurs gynécologiques et du développement (927)|
Prévention du cancer du col utérin (933)
|VIVIANO, Manuela et al. A comparison of cotton and flocked swabs for vaginal self-sample collection. In: International Journal of Women's Health, 2018, vol. Volume 10, p. 229-236. doi: 10.2147/IJWH.S157897 https://archive-ouverte.unige.ch/unige:105995|