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Scientific article
English

Influence of lipophilicity and stereochemistry at the C-7 position on the cardioprotective and antioxidant effect of ginkgolides during rat heart ischemia and reperfusion

Published inDrug development research, vol. 64, no. 3, p. 157-171
Publication date2005
Abstract

The extent to which the cardioprotective effect of ginkgolides is related to their lipophilicity rather than to their anti-platelet activating factor (PAF) effect was addressed in isolated rat hearts submitted to ischemia and reperfusion. A new derivative of ginkgolide C (1), the 7-alpha-O-(4-methylphenyl) ginkgolide C (4) was synthesized and compared to 7-O-(4-n-methylphenyl) ginkgolide C (2) that had the same absolute configuration at C-7 as 1 for its lipophilicity, anti-PAF activity, and cardioprotective and antioxidant effects. Using reversed-phase high-performance liquid chromatography HPLC, 4 and 2 were found to be significantly more lipophilic (i.e., log k(w) of 3.42 +/- 0.05 and 3.64 +/- 0.07, respectively) than 1 (1.15 +/- 0.03) and the strong PAF inhibitor ginkgolide B (GkB; 1.65 +/- 0.03). The anti-PAF activities (IC50 values in mu M) were 8.2, 17.1, and 2.2 for 4, 1, and GkB, respectively, while 2 was inactive. In preischemic and/or reperfused hearts perfused with ginkgolides at 0.7 mu M: (i) 2 and 4 were more efficient in improving postischemic hemodynamic and metabolic recovery than 1, (ii) a key-step in cardioprotection Occurred during ischemia where 2 and 4 limited myocardial ATP depletion and contracture development, (iii) a strong anti-lipoperoxidant effect was observed with 2 and 4, but not 1. In vivo administration of 2 to rats (4 mg/kg/day for 20 days) was more effective than that of I regarding ischemic heart protection, suggesting a positive role for lipophilicity. It was concluded that a high lipophilicity is not an absolute prerequisite for a strong anti-PAF effect for ginkgolides, whereas it appears essential for cardioprotection. (c) 2005 Wiley-Liss, Inc

Citation (ISO format)
BILLOTTET, L. et al. Influence of lipophilicity and stereochemistry at the C-7 position on the cardioprotective and antioxidant effect of ginkgolides during rat heart ischemia and reperfusion. In: Drug development research, 2005, vol. 64, n° 3, p. 157–171.
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  • PID : unige:10535
ISSN of the journal0272-4391
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