Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma

Donahue, Mark P.; Rose, Keith; Hochstrasser, Denis; Vonderscher, Jacky Pierre Michel; Grass, Peter; Chibout, Salah-Dine; Nelson, Charlotte L.; Sinnaeve, Peter; Goldschmidt-Clermont, Pascal J.; Granger, Christopher B.

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Title		Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma
Authors		Donahue, Mark P. Rose, Keith Hochstrasser, Denis Vonderscher, Jacky Pierre Michel Grass, Peter Chibout, Salah-Dine Nelson, Charlotte L. Sinnaeve, Peter Goldschmidt-Clermont, Pascal J. Granger, Christopher B. show all authors [1 - 10]
Published in		American Heart Journal. 2006, vol. 152, no. 3, p. 478-485
Abstract		Background Relating a disease state to an entire population of proteins provides an opportunity to gain new insights into a disease. Methods Male populations of 53 patients with angiographic coronary artery disease and 53 control subjects without coronary disease from the Duke Databank for Cardiovascular Disease were established and matched for age and race as well as extremes of risk factors. Major plasma protein abnormalities were excluded. Plasma samples of each group were pooled to make large volumes (6 L each) to identify low-abundance proteins. After removal of albumin as well as immunoglobulins and enrichment of smaller proteins (<20-40 kDa), samples were separated into 12 960 fractions by cation exchange and 2 reversed-phase chromatography steps. Proteins were analyzed by liquid chromatography–electrospray ionization tandem mass spectrometry. Results There were 731 plasma proteins or fragments identified. Of these proteins, 95 were differentially displayed in the case versus control populations. These represent broad categories of proteins involved with natural defenses, inflammation, growth, and coagulation.
Identifiers		DOI: 10.1016/j.ahj.2006.03.007
Full text		Article (Published version) (188 Kb) - Limited access to UNIGE Other version: http://linkinghub.elsevier.com/retrieve/pii/S0002870306002328
Structures		Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Citation (ISO format)		DONAHUE, Mark P. et al. Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma. In: American Heart Journal, 2006, vol. 152, n° 3, p. 478-485. https://archive-ouverte.unige.ch/unige:103666