Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma

Article Article scientifique Article Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma fre 2006 author Donahue Mark P. author Rose Keith author Hochstrasser Denis 26422 author Vonderscher Jacky Pierre Michel 76298 author Grass Peter author Chibout Salah-Dine author Nelson Charlotte L. author Sinnaeve Peter author Goldschmidt-Clermont Pascal J. author Granger Christopher B. 478-485 10.1016/j.ahj.2006.03.007 0002-8703 http://linkinghub.elsevier.com/retrieve/pii/S0002870306002328 Background Relating a disease state to an entire population of proteins provides an opportunity to gain new insights into a disease. Methods Male populations of 53 patients with angiographic coronary artery disease and 53 control subjects without coronary disease from the Duke Databank for Cardiovascular Disease were established and matched for age and race as well as extremes of risk factors. Major plasma protein abnormalities were excluded. Plasma samples of each group were pooled to make large volumes (6 L each) to identify low-abundance proteins. After removal of albumin as well as immunoglobulins and enrichment of smaller proteins (<20-40 kDa), samples were separated into 12 960 fractions by cation exchange and 2 reversed-phase chromatography steps. Proteins were analyzed by liquid chromatography–electrospray ionization tandem mass spectrometry. Results There were 731 plasma proteins or fragments identified. Of these proteins, 95 were differentially displayed in the case versus control populations. These represent broad categories of proteins involved with natural defenses, inflammation, growth, and coagulation. Dewey 576 136 Département de biologie structurale et bioinformatique 21E false American Heart Journal 152 3 1b21a56d-9fd7-4419-a52c-c4cdffcbfeb2 Article (Published version) Restricted 1-s2.0-S0002870306002328-main.pdf 192172 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Discovery of proteins related to coronary artery disease using industrial-scale proteomics analysis of pooled plasma	American Heart Journal			2006	396	0

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Vonderscher, Jacky Pierre Michel