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Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future

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Published in American Pharmaceutical Review. 2018, p. 1-4
Abstract Size exclusion chromatography (SEC) is a historical technique, routinely applied for the separation of species possessing different molecular masses (sizes). It is considered as a reference method for the qualitative and quantitative analysis of protein aggregates. In the last few years,several improvements were brought to high performance SEC (HP-SEC or conventional SEC) in terms of particle size, inertness and column dimension. Today, SEC columns of smaller dimensions packed with sub-3 μm particles (also known as ultra-high performance SEC (UHPSEC)) are used, to obtain faster and better separation compared to HP-SEC. This paper discusses the possibilities and limitations of both HP-SEC and UHP-SEC and also describes some possible directions for future applications related to protein biopharmaceuticals analysis.
Keywords BiopharmaceuticalsSize-exclusion chromatographyAggregateMonoclonalAntibodyAntibody drug conjugateFusion proteins
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FEKETE, Szabolcs et al. Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future. In: American Pharmaceutical Review, 2018, p. 1-4. https://archive-ouverte.unige.ch/unige:103247

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Deposited on : 2018-03-28

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