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Scientific article
Open access
English

Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future

Published inAmerican Pharmaceutical Review, p. 1-4
Publication date2018
Abstract

Size exclusion chromatography (SEC) is a historical technique, routinely applied for the separation of species possessing different molecular masses (sizes). It is considered as a reference method for the qualitative and quantitative analysis of protein aggregates. In the last few years,several improvements were brought to high performance SEC (HP-SEC or conventional SEC) in terms of particle size, inertness and column dimension. Today, SEC columns of smaller dimensions packed with sub-3 μm particles (also known as ultra-high performance SEC (UHPSEC)) are used, to obtain faster and better separation compared to HP-SEC. This paper discusses the possibilities and limitations of both HP-SEC and UHP-SEC and also describes some possible directions for future applications related to protein biopharmaceuticals analysis.

Keywords
  • Biopharmaceuticals
  • Size-exclusion chromatography
  • Aggregate
  • Monoclonal
  • Antibody
  • Antibody drug conjugate
  • Fusion proteins
Citation (ISO format)
FEKETE, Szabolcs et al. Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future. In: American Pharmaceutical Review, 2018, p. 1–4.
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Article (Published version)
accessLevelPublic
Identifiers
  • PID : unige:103247
ISSN of the journal1099-8012
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Technical informations

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