Scientific article
OA Policy
English

Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future

Published inAmerican Pharmaceutical Review, p. 1-4
Publication date2018
Abstract

Size exclusion chromatography (SEC) is a historical technique, routinely applied for the separation of species possessing different molecular masses (sizes). It is considered as a reference method for the qualitative and quantitative analysis of protein aggregates. In the last few years,several improvements were brought to high performance SEC (HP-SEC or conventional SEC) in terms of particle size, inertness and column dimension. Today, SEC columns of smaller dimensions packed with sub-3 μm particles (also known as ultra-high performance SEC (UHPSEC)) are used, to obtain faster and better separation compared to HP-SEC. This paper discusses the possibilities and limitations of both HP-SEC and UHP-SEC and also describes some possible directions for future applications related to protein biopharmaceuticals analysis.

Keywords
  • Biopharmaceuticals
  • Size-exclusion chromatography
  • Aggregate
  • Monoclonal
  • Antibody
  • Antibody drug conjugate
  • Fusion proteins
Research groups
Citation (ISO format)
FEKETE, Szabolcs et al. Size Exclusion Chromatography of Protein Biopharmaceuticals: Past, Present and Future. In: American Pharmaceutical Review, 2018, p. 1–4.
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
  • PID : unige:103247
Journal ISSN1099-8012
1365views
1303downloads

Technical informations

Creation14/03/2018 11:18:00
First validation14/03/2018 11:18:00
Update time15/03/2023 08:01:54
Status update15/03/2023 08:01:53
Last indexation31/10/2024 09:55:29
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack