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Scientific article
Open access
English

A pathway of targeted autophagy is induced by DNA damage in budding yeast

Published inProceedings of the National Academy of Sciences, vol. 114, no. 7, p. E1158-E1167
Publication date2017
Abstract

Autophagy plays a central role in the DNA damage response (DDR) by controlling the levels of various DNA repair and checkpoint proteins; however, how the DDR communicates with the autophagy pathway remains unknown. Using budding yeast, we demonstrate that global genotoxic damage or even a single unrepaired double-strand break (DSB) initiates a previously undescribed and selective pathway of autophagy that we term genotoxin-induced targeted autophagy (GTA). GTA requires the action primarily of Mec1/ATR and Rad53/CHEK2 checkpoint kinases, in part via transcriptional up-regulation of central autophagy proteins. GTA is distinct from starvation-induced autophagy. GTA requires Atg11, a central component of the selective autophagy machinery, but is different from previously described autophagy pathways. By screening a collection of ∼6,000 yeast mutants, we identified genes that control GTA but do not significantly affect rapamycin-induced autophagy. Overall, our findings establish a pathway of autophagy specific to the DNA damage response.

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Citation (ISO format)
EAPEN, Vinay V et al. A pathway of targeted autophagy is induced by DNA damage in budding yeast. In: Proceedings of the National Academy of Sciences, 2017, vol. 114, n° 7, p. E1158–E1167. doi: 10.1073/pnas.1614364114
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ISSN of the journal0027-8424
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