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Master
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1st project: The Identification of the Mitochondrial Inner Membrane Alanine Carrier. 2nd project: The Role of the Malate-Aspartate Shuttle in the Cancer Cell's Metabolism

ContributorsOta, Marina
Master program titleBiologie
Defense date2018
Abstract

Alanine is known to compensate the lack of pyruvate in mitochondrial pyruvate carrier deleted mitochondria using its own unknown carrier. We searched the gene coding for this carrier. A screen was done using a yeast library in which each yeast carried a single gene deletion on selective media. The result did not give the gene coding for the carrier itself but allowed us to find some interesting genes involved in the alanine transport. A deregulation of the mitochondrial metabolism in cancer cells is frequent. The malate-aspartate shuttle was observed to play a role in cancer development. We investigate the function of the malate-aspartate shuttle in human cancer cell line. We knocked-down ARALAR1 using shRNA and experiments were done to see its effect on the proliferation, the mitochondrial morphology and mitochondrial metabolism in these cells. The results did not show a significant disruption of proliferation, mitochondrial morphology and metabolism.

eng
Keywords
  • Mitochondria
  • Mitochondrial pyruvate carrier
  • Alanine transport
  • Yeast
  • Malate-aspartate shuttle
  • Cancer
Research group
Citation (ISO format)
OTA, Marina. 1st project: The Identification of the Mitochondrial Inner Membrane Alanine Carrier. 2nd project: The Role of the Malate-Aspartate Shuttle in the Cancer Cell’s Metabolism. 2018.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:102604
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Creation02/28/2018 1:00:00 AM
First validation02/28/2018 1:00:00 AM
Update time03/15/2023 7:55:16 AM
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