1st project: The Identification of the Mitochondrial Inner Membrane Alanine Carrier. 2nd project: The Role of the Malate-Aspartate Shuttle in the Cancer Cell's Metabolism

Diplôme Master 1st project: The Identification of the Mitochondrial Inner Membrane Alanine Carrier. 2nd project: The Role of the Malate-Aspartate Shuttle in the Cancer Cell's Metabolism eng 2018 author Ota Marina 723939 director Martinou Jean-Claude 27293 Alanine is known to compensate the lack of pyruvate in mitochondrial pyruvate carrier deleted mitochondria using its own unknown carrier. We searched the gene coding for this carrier. A screen was done using a yeast library in which each yeast carried a single gene deletion on selective media. The result did not give the gene coding for the carrier itself but allowed us to find some interesting genes involved in the alanine transport. A deregulation of the mitochondrial metabolism in cancer cells is frequent. The malate-aspartate shuttle was observed to play a role in cancer development. We investigate the function of the malate-aspartate shuttle in human cancer cell line. We knocked-down ARALAR1 using shRNA and experiments were done to see its effect on the proliferation, the mitochondrial morphology and mitochondrial metabolism in these cells. The results did not show a significant disruption of proliferation, mitochondrial morphology and metabolism. Mitochondria Mitochondrial pyruvate carrier Alanine transport Yeast Malate-aspartate shuttle Cancer Dewey 570 Biologie 46 Département de biologie cellulaire 165 false 837 Groupe Martinou 9b18a6d3-5217-4a3e-a8f1-c43060151df8 Master thesis Restricted Master.pdf 8977921 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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1st project: The Identification of the Mitochondrial Inner Membrane Alanine Carrier. 2nd project: The Role of the Malate-Aspartate Shuttle in the Cancer Cell's Metabolism				2018	204	17

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Ota, Marina