en
Scientific article
English

An ether -a-go-go K+ current, Ih-eag, contributes to the hyperpolarization of human fusion-competent myoblasts

Published inJournal of physiology, vol. 512, no. 2, p. 317-323
Publication date1998
Abstract

1. Two early signs of human myoblast commitment to fusion are membrane potential hyperpolarization and concomitant expression of a non-inactivating delayed rectifier K+ current, IK(NI). This current closely resembles the outward K+ current elicited by rat ether-a-go-go (r-eag) channels in its range of potential for activation and unitary conductance. 2. It is shown that activation kinetics of IK(NI), like those of r-eag, depend on holding potential and on [Mg2+]o, and that IK(NI), like r-eag, is reversibly inhibited by a rise in [Ca2+]i. 3. Forced expression of an isolated human ether-a-go-go K+ channel (h-eag) cDNA in undifferentiated myoblasts generates single-channel and whole-cell currents with remarkable similarity to IK(NI). 4. h-eag current (Ih-eag) is reversibly inhibited by a rise in [Ca2+]i, and the activation kinetics depend on holding potential and [Mg2+]o. 5. Forced expression of h-eag hyperpolarizes undifferentiated myoblasts from -9 to -50 mV, the threshold for the activation of both Ih-eag and IK(NI). Similarly, the higher the density of IK(NI), the more hyperpolarized the resting potential of fusion-competent myoblasts. 6. It is concluded that h-eag constitutes the channel underlying IK(NI) and that it contributes to the hyperpolarization of fusion-competent myoblasts. To our knowledge, this is the first demonstration of a physiological role for a mammalian eag K+ channel.

Keywords
  • Adolescent
  • Algorithms
  • Animals
  • Calcium/metabolism
  • Cell Differentiation/physiology
  • Cell Fusion/physiology
  • Cell Polarity/ physiology
  • Cells, Cultured
  • Child
  • Child, Preschool
  • Electric Stimulation
  • Electrophysiology
  • Ether-A-Go-Go Potassium Channels
  • Humans
  • Infant
  • Membrane Potentials/physiology
  • Muscle Development
  • Muscle, Skeletal/cytology/growth & development/ metabolism
  • Patch-Clamp Techniques
  • Potassium Channels/ metabolism
  • Rats
Citation (ISO format)
BIJLENGA, P. et al. An ether -a-go-go K+ current, Ih-eag, contributes to the hyperpolarization of human fusion-competent myoblasts. In: Journal of physiology, 1998, vol. 512, n° 2, p. 317–323. doi: 10.1111/j.1469-7793.1998.317be.x
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0022-3751
489views
0downloads

Technical informations

Creation08/06/2010 1:46:04 PM
First validation08/06/2010 1:46:04 PM
Update time03/14/2023 3:57:54 PM
Status update03/14/2023 3:57:53 PM
Last indexation02/12/2024 7:01:35 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack