• Home
  1. Home
  2. Contribution of a non-inactivating potassium current to the resting membrane potential of fusion-competent human myoblasts
Scientific article
English

Contribution of a non-inactivating potassium current to the resting membrane potential of fusion-competent human myoblasts

Contributors

Bernheim, Laurent; Liu, J. H.; Hamann, M.; Haenggeli, Charles-Antoine; Fischer-Lougheed, Jacqueline; Bader, Charles

Published in

Journal of physiology, vol. 493, no. 1, p. 129-141

Publication date

1996

Abstract

1. Using the patch-clamp technique, a new non-inactivating voltage-gated potassium current, IK(ni), was studied in cultured fusion-competent human myoblasts. 2. IK(ni) is activated at voltages above -50 mV and its conductance reaches its maximum around +50 mV. Once activated, the current remains at a steady level for minutes. 3. Reversal potential measurements at various extracellular potassium concentrations indicate that potassium ions are the major charge carriers of IK(ni). 4. IK(ni) is insensitive to potassium channel blockers such as charybdotoxin, dendrotoxins, mast cell degranulating (MCD) peptide, 4-aminopyridine (4-AP), 3,4-diaminopyridine (3,4-DAP) and apamin, but can be blocked by high concentrations of TEA and by Ba2+. 5. A potassium channel of small conductance (8.4 pS at +40 mV) with potential dependence and pharmacological properties corresponding to those of IK(ni) in whole-cell recording is described. 6. IK(ni) participates in the control of the resting potential of fusion-competent myoblasts, suggesting that it may play a key role in the process of myoblast fusion.

Keywords

  • Adolescent
  • Adult
  • Aminopyridines/pharmacology
  • Cell Fusion/ physiology
  • Cells, Cultured
  • Charybdotoxin/pharmacology
  • Child
  • Child, Preschool
  • Elapid Venoms/pharmacology
  • Electrophysiology
  • Humans
  • Infant
  • Membrane Potentials/ physiology
  • Muscle, Skeletal/cytology/ metabolism
  • Patch-Clamp Techniques
  • Peptides/pharmacology
  • Potassium/ metabolism
  • Potassium Channel Blockers
  • Potassium Channels/ metabolism

UNIGE affiliation entities

Citation (ISO format)

BERNHEIM, Laurent et al. Contribution of a non-inactivating potassium current to the resting membrane potential of fusion-competent human myoblasts. In: Journal of physiology, 1996, vol. 493, n° 1, p. 129–141. doi: 10.1113/jphysiol.1996.sp021369

Main files (1)

Article

Identifiers

Additional URL for this publication

http://jp.physoc.org/content/493/Pt_1/129.abstract

Journal ISSN

0022-3751
719views
0downloads

Technical informations

Creation

06/08/2010 13:46:03

First validation

06/08/2010 13:46:03

Update

14/03/2023 15:57:53

Status update

14/03/2023 15:57:52

Last indexation

29/10/2024 16:27:14
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated