Scientific article

Oligodendrocyte progenitor migration in response to injury of glial monolayers requires the polysialic neural cell-adhesion molecule

Published inJournal of neuroscience research, vol. 72, no. 6, p. 679-690
Publication date2003

Injury to the nervous system results in reactive astrogliosis that is a critical determinant of neuronal regeneration. To analyze glial responses to mechanical injury and the role of the polysialic neural cell adhesion molecule (PSA-NCAM) in this process, we established primary glia cultures from newborn rat cerebral cortex. Scratching a confluent monolayer of primary glial cells resulted in two major events: rapid migration of oligodendrocyte progenitor-like (O-2A) cells into the wounded area and development of polarized morphology of type 1 astrocytes at the wound edge. Migrating O-2A progenitors had a bipolar morphology and exhibited A2B5 and O4 immunolabeling. Once these cells were established inside the wounded area, they lost A2B5 immunoreactivity and differentiated into glial fibrillary acidic protein-positive astrocytes. Migrating O-2A cells expressed PSA-NCAM, but type 1 astrocytes at the wound edge did not. Treatment of wounded cultures with Endo-N, which specifically removes PSA from the surface of cells, resulted in a significant decrease in O-2A cell migration into the wounded area and completely blocked the wound closure. Video time-lapse analysis showed that, in the presence of Endo-N, O-2A cells remained motile and migrated short distances but did not move away from the monolayer. These results demonstrate that O-2A progenitors contribute to reactive astrogliosis in culture and that PSA-NCAM is involved in this process by regulating cell migration.

  • Animals
  • Cell Movement/ physiology
  • Cells, Cultured
  • Cerebral Cortex/cytology/metabolism
  • Gliosis/metabolism
  • Neural Cell Adhesion Molecule L1/ biosynthesis
  • Neuroglia/cytology/ metabolism
  • Oligodendroglia/ cytology/ metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sialic Acids/ biosynthesis
  • Stem Cells/ cytology/ metabolism
Citation (ISO format)
BARRAL-MORAN, M. J. et al. Oligodendrocyte progenitor migration in response to injury of glial monolayers requires the polysialic neural cell-adhesion molecule. In: Journal of neuroscience research, 2003, vol. 72, n° 6, p. 679–690. doi: 10.1002/jnr.10627
Updates (1)
ISSN of the journal0360-4012

Technical informations

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