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Oligodendrocyte progenitor migration in response to injury of glial monolayers requires the polysialic neural cell-adhesion molecule

Barral-Moran, M. J.
Calaora, V.
Wang, C.
Durbec, Pascale
Rougon, G.
Published in Journal of neuroscience research. 2003, vol. 72, no. 6, p. 679-690
Abstract Injury to the nervous system results in reactive astrogliosis that is a critical determinant of neuronal regeneration. To analyze glial responses to mechanical injury and the role of the polysialic neural cell adhesion molecule (PSA-NCAM) in this process, we established primary glia cultures from newborn rat cerebral cortex. Scratching a confluent monolayer of primary glial cells resulted in two major events: rapid migration of oligodendrocyte progenitor-like (O-2A) cells into the wounded area and development of polarized morphology of type 1 astrocytes at the wound edge. Migrating O-2A progenitors had a bipolar morphology and exhibited A2B5 and O4 immunolabeling. Once these cells were established inside the wounded area, they lost A2B5 immunoreactivity and differentiated into glial fibrillary acidic protein-positive astrocytes. Migrating O-2A cells expressed PSA-NCAM, but type 1 astrocytes at the wound edge did not. Treatment of wounded cultures with Endo-N, which specifically removes PSA from the surface of cells, resulted in a significant decrease in O-2A cell migration into the wounded area and completely blocked the wound closure. Video time-lapse analysis showed that, in the presence of Endo-N, O-2A cells remained motile and migrated short distances but did not move away from the monolayer. These results demonstrate that O-2A progenitors contribute to reactive astrogliosis in culture and that PSA-NCAM is involved in this process by regulating cell migration.
Keywords AnimalsCell Movement/ physiologyCells, CulturedCerebral Cortex/cytology/metabolismGliosis/metabolismNeural Cell Adhesion Molecule L1/ biosynthesisNeuroglia/cytology/ metabolismOligodendroglia/ cytology/ metabolismRatsRats, Sprague-DawleySialic Acids/ biosynthesisStem Cells/ cytology/ metabolism
PMID: 12774308
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Research group Progéniteurs neuronaux
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BARRAL-MORAN, M. J. et al. Oligodendrocyte progenitor migration in response to injury of glial monolayers requires the polysialic neural cell-adhesion molecule. In: Journal of neuroscience research, 2003, vol. 72, n° 6, p. 679-690. doi: 10.1002/jnr.10627 https://archive-ouverte.unige.ch/unige:10173

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Deposited on : 2010-08-06

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