UNIGE document Scientific Article
previous document  unige:101703  next document
add to browser collection
Title

Impact of cycling cells and cell cycle regulation on Hydra regeneration

Authors
Published in Developmental Biology. 2018, vol. 433, no. 2, p. 240-253
Abstract Hydra tissues are made from three distinct populations of stem cells that continuously cycle and pause in G2 instead of G1. To characterize the role of cell proliferation after mid-gastric bisection, we have (i) used flow cytometry and classical markers to monitor cell cycle modulations, (ii) quantified the transcriptomic regulations of 202 genes associated with cell proliferation during head and foot regeneration, and (iii) compared the impact of anti-proliferative treatments on regeneration efficiency. We confirm two previously reported events: an early mitotic wave in head-regenerating tips, when few cell cycle genes are up-regulated, and an early-late wave of proliferation on the second day, preceded by the up-regulation of 17 cell cycle genes. These regulations appear more intense after mid-gastric bisection than after decapitation, suggesting a position-dependent regulation of cell proliferation during head regeneration. Hydroxyurea, which blocks S-phase progression, delays head regeneration when applied before but not after bisection. This result is consistent with the fact that the Hydra central region is enriched in G2-paused adult stem cells, poised to divide upon injury, thus forming a necessary constitutive pro-blastema. However a prolonged exposure to hydroxyurea does not block regeneration as cells can differentiate apical structures without traversing S-phase, and also escape in few days the hydroxyureainduced S-phase blockade. Thus Hydra head regeneration, which is a fast event, is highly plastic, relying on large stocks of adult stem cells paused in G2 at amputation time, which immediately divide to proliferate and/or differentiate apical structures even when S-phase is blocked.
Keywords Hydra regenerationCell cycling genesS-phase arrestHydroxyureaNocodazoleRNA-seq transcriptomicsFlow cytometryDNA profilingPro-blastema
Identifiers
PMID: 29291976
Full text
Structures
Projects FNS: 31003A_149630
FNS: 31003_169930
Citation
(ISO format)
BUZGARIU, Wanda Christa et al. Impact of cycling cells and cell cycle regulation on Hydra regeneration. In: Developmental Biology, 2018, vol. 433, n° 2, p. 240-253. https://archive-ouverte.unige.ch/unige:101703

118 hits

17 downloads

Update

Deposited on : 2018-01-29

Export document
Format :
Citation style :