Impact of cycling cells and cell cycle regulation on Hydra regeneration

Article Article scientifique Article Impact of cycling cells and cell cycle regulation on Hydra regeneration eng 2018 author Buzgariu Wanda Christa 642187 author Wenger Yvan 515193 author Tcaciuc Nina 854846 author Catunda Lemos Ana Paula 732930 author Galliot Brigitte 0000-0001-7596-8284 29979 240-253 10.1016/j.ydbio.2017.11.003 29291976 0012-1606 http://linkinghub.elsevier.com/retrieve/pii/S001216061730413X Hydra tissues are made from three distinct populations of stem cells that continuously cycle and pause in G2 instead of G1. To characterize the role of cell proliferation after mid-gastric bisection, we have (i) used flow cytometry and classical markers to monitor cell cycle modulations, (ii) quantified the transcriptomic regulations of 202 genes associated with cell proliferation during head and foot regeneration, and (iii) compared the impact of anti-proliferative treatments on regeneration efficiency. We confirm two previously reported events: an early mitotic wave in head-regenerating tips, when few cell cycle genes are up-regulated, and an early-late wave of proliferation on the second day, preceded by the up-regulation of 17 cell cycle genes. These regulations appear more intense after mid-gastric bisection than after decapitation, suggesting a position-dependent regulation of cell proliferation during head regeneration. Hydroxyurea, which blocks S-phase progression, delays head regeneration when applied before but not after bisection. This result is consistent with the fact that the Hydra central region is enriched in G2-paused adult stem cells, poised to divide upon injury, thus forming a necessary constitutive pro-blastema. However a prolonged exposure to hydroxyurea does not block regeneration as cells can differentiate apical structures without traversing S-phase, and also escape in few days the hydroxyureainduced S-phase blockade. Thus Hydra head regeneration, which is a fast event, is highly plastic, relying on large stocks of adult stem cells paused in G2 at amputation time, which immediately divide to proliferate and/or differentiate apical structures even when S-phase is blocked. Hydra regeneration Cell cycling genes S-phase arrest Hydroxyurea Nocodazole RNA-seq transcriptomics Flow cytometry DNA profiling Pro-blastema Dewey 590 Swiss National Science Foundation Regulation of stem cell plasticity in Hydra 149630 CH Project funding Swiss National Science Foundation 31003_169930 CH 44 Département de génétique et évolution 162 false https://www.sciencedirect.com/science/article/pii/S001216061730413X?via%3Dihub Developmental Biology 433 2 0f428c0b-7b06-482a-8da0-ae1eb7d55208 Article (Published version) Public 2018_Buzgariu_Hydra-Cell-cycling_DevBiol.pdf 2313998 application/pdf CC BY-NC-ND openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Impact of cycling cells and cell cycle regulation on Hydra regeneration	Developmental Biology			2018	605	414

Archive ouverte UNIGE

Catunda Lemos, Ana Paula