Distribution and projections of cholecystokinin-immunoreactive neurons in the hypothalamic paraventricular nucleus of rat
|Published in||Journal of Comparative Neurology. 1984, vol. 227, no. 2, p. 173-181|
|Abstract||Analysis of coronal sections from colchicine-treated rat brains reveals that CCK-immunoreactivity (CCK-ir) is present in two distinguishable neuronal systems in the paraventricular nucleus (PVN). More than 60% of these cells were found to be typical parvicellular neurons; the remainder were magnocellular neurons. The magnocellular CCK-ir neurons were concentrated in the medial magnocellular subdivision, while more caudally they formed a ring around a zone of unstained magnocellular neurons. Immunostained parvicellular neurons predominate in medial and periventricular parvicellular subdivisions. The efferent projections of CCK-ir neurons were investigated by looking for retrograde accumulation of CCK-ir in cell bodies after selective knife cuts. A parasagittal cut of the lateral retrochiasmatic area as well as transection of the rostral median eminence resulted in an accumulation of CCK-ir material in a large number of both parvi- and magnocellular neurons. After pituitary stalk lesions, however, increased staining was only seen in magnocellular neurons. It is inferred that the magnocellular (presumed oxytocin-CCK) cells send their axons to the pituitary, whereas axons of CCK-ir parvicellular neurons appear to terminate in the median eminence. After transection of the medial forebrain bundle (MFB), immunostaining increased in a small number of scattered transected fibers proximal to the knife cut and in a few perikarya in the PVN, indicating that very few CCK cells may send descending fibers to the lower brainstem.|
|Keywords||Animals — Brain Mapping — Brain Stem/anatomy & histology — Cholecystokinin/ metabolism — Immunoenzyme Techniques — Male — Medial Forebrain Bundle/anatomy & histology — Median Eminence/anatomy & histology — Neural Pathways/anatomy & histology — Paraventricular Hypothalamic Nucleus/anatomy & histology/ metabolism — Rats — Rats, Inbred Strains|
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|Research group||Progéniteurs neuronaux|
|KISS, Jozsef Zoltan, WILLIAMS, T. H., PALKOVITS, M. Distribution and projections of cholecystokinin-immunoreactive neurons in the hypothalamic paraventricular nucleus of rat. In: Journal of Comparative Neurology, 1984, vol. 227, n° 2, p. 173-181. doi: 10.1002/cne.902270204 https://archive-ouverte.unige.ch/unige:10083|