en
Scientific article
English

An improved method for Lowicryl K4M electron microscopic embedding of brain tissue

Published inJournal of neuroscience methods, vol. 30, no. 1, p. 29-32
Publication date1989
Abstract

The Lowicryl K4M low-temperature embedding technique was modified for brain tissue to facilitate antigen localization in neuronal structures by using postembedding immunoelectron microscopy. Rat brains fixed by perfusion with a mixture of 1% glutaraldehyde and 1% paraformaldehyde, were sectioned into 200-microns-thick coronal sections, and the appropriate area of the sections further microdissected into small samples (200 x 700 x 700 microns). These samples were processed for K4M embedding using prolonged incubation times at each step. This resulted in a homogeneously infiltrated tissue with good preservation of ultrastructure and antigen reactivity. Despite the lack of osmium fixation, the morphology of the synapses was clearly identifiable.

Keywords
  • Acrylic Resins
  • Animals
  • Brain/metabolism/ ultrastructure
  • Immunohistochemistry/ methods
  • Male
  • Microscopy, Electron/ methods
  • Microtomy/ methods
  • Neurotransmitter Agents/ metabolism
  • Rats
Citation (ISO format)
KISS, Jozsef Zoltan. An improved method for Lowicryl K4M electron microscopic embedding of brain tissue. In: Journal of neuroscience methods, 1989, vol. 30, n° 1, p. 29–32. doi: 10.1016/0165-0270(89)90071-X
Identifiers
ISSN of the journal0165-0270
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