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Scientific article
English

An improved method for Lowicryl K4M electron microscopic embedding of brain tissue

Contributors

Kiss, Jozsef Zoltan

Published in

Journal of neuroscience methods, vol. 30, no. 1, p. 29-32

Publication date

1989

Abstract

The Lowicryl K4M low-temperature embedding technique was modified for brain tissue to facilitate antigen localization in neuronal structures by using postembedding immunoelectron microscopy. Rat brains fixed by perfusion with a mixture of 1% glutaraldehyde and 1% paraformaldehyde, were sectioned into 200-microns-thick coronal sections, and the appropriate area of the sections further microdissected into small samples (200 x 700 x 700 microns). These samples were processed for K4M embedding using prolonged incubation times at each step. This resulted in a homogeneously infiltrated tissue with good preservation of ultrastructure and antigen reactivity. Despite the lack of osmium fixation, the morphology of the synapses was clearly identifiable.

Keywords

  • Acrylic Resins
  • Animals
  • Brain/metabolism/ ultrastructure
  • Immunohistochemistry/ methods
  • Male
  • Microscopy, Electron/ methods
  • Microtomy/ methods
  • Neurotransmitter Agents/ metabolism
  • Rats

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

KISS, Jozsef Zoltan. An improved method for Lowicryl K4M electron microscopic embedding of brain tissue. In: Journal of neuroscience methods, 1989, vol. 30, n° 1, p. 29–32. doi: 10.1016/0165-0270(89)90071-X

Identifiers

Additional URL for this publication

http://www.sciencedirect.com/science/article/pii/016502708990071X

Journal ISSN

0165-0270
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Technical informations

Creation

06/08/2010 10:47:13

First validation

06/08/2010 10:47:13

Update

14/03/2023 15:57:25

Status update

14/03/2023 15:57:25

Last indexation

29/10/2024 16:24:21
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